Controlled delivery of proteins into bilayer lipid membranes on chip.
Controlled delivery of proteins into bilayer lipid membranes on chip.
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DOI：
10.1039/b703818f
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发表时间：
2007-09
期刊：
影响因子：
6.1
通讯作者：
Morgan, Hywel
中科院分区：
文献类型：
--
作者：
Zagnoni, Michele;Sandison, Mairi E.;Marius, Phedra;Lee, Anthony G.;Morgan, Hywel
用于药物筛选应用的膜蛋白的研究和开发需要一种可控且可靠的方法将其递送到基于芯片实验室技术的人工悬浮膜平台中。在这项工作中，提出了一种用于形成适合电生理学研究和生物传感器应用的脂质双层的聚合物装置。该芯片支持单个双层，并被配置为通过芯片上微流体进行受控蛋白质递送。为了证明蛋白质递送的原理，将钾通道KcsA重构为蛋白脂质体，然后将其与芯片上的悬浮双层融合。电鉴定单个脂蛋白体与膜的融合。单通道电导测量的KcsA在芯片上的双层进行了记录，这些进行了比较，以前发表的数据与传统的平面双层系统。
The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.
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