Kaempferol attenuates cognitive deficit via regulating oxidative stress and neuroinflammation in an ovariectomized rat model of sporadic dementia.
Kaempferol attenuates cognitive deficit via regulating oxidative stress and neuroinflammation in an ovariectomized rat model of sporadic dementia.
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DOI：
10.4103/1673-5374.238714
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发表时间：
2018-10
影响因子：
6.1
通讯作者：
Babaei P
中科院分区：
文献类型：
--
作者：
Kouhestani S;Jafari A;Babaei P
阿尔茨海默病（AD）与氧化应激有关，并最终导致认知缺陷。尽管已有关于AD病理生理学的文献，但目前尚无治愈AD的方法。研究了从德国麦穗草提取物中提取的山萘酚（Kmp）对小鼠的促生长作用。（3）叶对散发性AD的卵巢切除（OVX）大鼠模型中的认知障碍、海马抗氧化剂、细胞凋亡、脂质过氧化和神经炎症标志物的影响。山奈酚是山奈提取物中主要的黄酮类化合物，具有抗氧化、抗炎和抗神经毒性作用。将32只雌性Wistar大鼠切除卵巢，随机分为假手术组、去卵巢+生理盐水组、去卵巢+链脲佐菌素（STZ）+生理盐水组、去卵巢+ STZ + Kmp组。侧脑室注射STZ（3 mg/kg，2次，间隔1d）建立散发性AD模型。OVX + STZ + Kmp组腹腔注射Kmp 10 mg/kg，连续21 d。采用Morris水迷宫评价大鼠的空间学习记忆能力。最后，脑匀浆用于酶联免疫吸附测定的生化分析。结果显示，与OVX + STZ组相比，OVX + STZ + Kmp组的空间学习和记忆能力明显改善，表现为Morris水迷宫中的逃避潜伏期和搜索距离缩短。Kmp还表现出显着升高脑超氧化物歧化酶和谷胱甘肽的抗氧化酶水平，而肿瘤坏死因子-α和丙二醛的减少。我们的研究结果表明，Kmp是能够减轻STZ诱导的记忆障碍OVX大鼠，可能是通过提高内源性海马抗氧化剂的超氧化物歧化酶和谷胱甘肽，并减少神经炎症。本研究提示Kmp可能是一种潜在的抗AD认知功能障碍的神经保护剂。
Alzheimer's disease (AD) is associated with oxidative stress, and ultimately results in cognitive deficit. Despite existing literature on the pathophysiology of AD, there is currently no cure for AD. The present study investigated the effects of kaempferol (Kmp) isolated from the extract of Mespilus germanica L. (medlar) leaves on cognitive impairment, hippocampal antioxidants, apoptosis, lipid peroxidation and neuro-inflammation markers in ovariectomized (OVX) rat models of sporadic AD. Kaempferol, as the main flavonoid of medlar extract has been previously known for anti-oxidative, anti-inflammatory and anti-neurotoxic effects. Thirty-two female Wistar rats were ovariectomized, and randomly divided into four groups: sham, OVX + saline, OVX + streptozotocin (STZ) + saline, OVX + STZ + Kmp. Animals received intracerebroventricular injection of STZ (3 mg/kg, twice with one day interval) to establish models of sporadic AD. Intraperitoneal injection of Kmp (10 mg/kg) for 21 days was performed in the OVX + STZ + Kmp group. Spatial learning and memory of rats were evaluated using a Morris water maze. Finally, brain homogenates were used for biochemical analysis by enzyme-linked immunosorbent assay. The results showed a significant improvement in spatial learning and memory as evidenced by shortened escape latency and searching distance in Morris water maze in the OVX + STZ + Kmp group compared with the OVX + STZ group. Kmp also exhibited significant elevations in brain levels of antioxidant enzymes of superoxide dismutase and glutathione, while reduction in tumor necrosis factor-α and malondialdehyde. Our results demonstrate that Kmp is capable of alleviating STZ-induced memory impairment in OVX rats, probably by elevating endogenous hippocampal antioxidants of superoxide dismutase and glutathione, and reducing neuroinflammation. This study suggests that Kmp may be a potential neuroprotective agent against cognitive deficit in AD.
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影响因子：
1.6
作者：
Kim, Jae Kyeom;Choi, Soo Jung;Shin, Dong-Hoon
通讯作者：
Shin, Dong-Hoon
DOI：
10.2174/138955708786369582
发表时间：
2008-11
期刊：
Mini reviews in medicinal chemistry
影响因子：
--
作者：
Aliev G;Obrenovich ME;Reddy VP;Shenk JC;Moreira PI;Nunomura A;Zhu X;Smith MA;Perry G
通讯作者：
Perry G
影响因子：
4.2
作者：
Kamat, Pradip K.;Rai, Shivika;Nath, Chandishwar
通讯作者：
Nath, Chandishwar
影响因子：
7.2
作者：
Parker WH;Broder MS;Chang E;Feskanich D;Farquhar C;Liu Z;Shoupe D;Berek JS;Hankinson S;Manson JE
通讯作者：
Manson JE
影响因子：
6.1
作者：
Kampkoetter, Andreas;Nkwonkam, Christiane Gombitang;Kahl, Regine
通讯作者：
Kahl, Regine