Geometric Angles and Gene Expression in Cells for Structural Bone Regeneration.
Geometric Angles and Gene Expression in Cells for Structural Bone Regeneration.
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DOI：
10.1002/advs.202304111
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发表时间：
2023-11
期刊：
影响因子：
15.1
通讯作者：
Cui, Wenguo
中科院分区：
文献类型：
--
作者：
Wang, Juan;Yang, Qianhao;Saiding, Qimanguli;Chen, Liang;Liu, Mingyue;Wang, Zhen;Xiang, Lei;Deng, Lianfu;Chen, Yixuan;Cui, Wenguo
几何和角度在细胞过程中起着至关重要的作用；然而，其监管机制仍不清楚。在这项研究中，我们利用近场静电打印技术构建了一系列具有不同几何形状的三维打印微纤维，以研究几何形状对干细胞功能和骨再生的调节机制。支架精确地模拟细胞尺寸，具有高孔隙率和互操作性。与其他空间形貌角度相比，具有90°拓扑结构的微纤维能显著促进骨髓间充质干细胞（BMSCs）成骨基因蛋白的表达。不同空间结构对骨髓间充质干细胞分化基因表达谱的影响是相关的，并通过microRNA测序进行验证。富集分析表明，90°微纤维通过显著上调miR‐222‐5p/cbfb/Runx2的表达，促进骨髓间充质干细胞成骨。使用颅骨缺损模型评估几何结构促进骨再生的能力，表明90°纤维支架显著促进新骨再生和新血管神经网络的形成。这项研究首次阐明了角度几何与细胞基因表达之间的关系，对理解几何结构如何促进干细胞分化、增殖和结构骨再生的功能有重要贡献。构建了一系列不同几何形状的微纤维，研究几何形状对干细胞功能和骨再生的调控机制。具有90°拓扑结构的微纤维模式可以通过上调miR‐222‐5p/cbfb/Runx2轴，有效促进间充质干细胞成骨基因蛋白的表达，加速新骨再生和神经血管网络的形成。
Geometry and angles play crucial roles in cellular processes; however, its mechanisms of regulation remain unclear. In this study, a series of three dimensional (3D)‐printed microfibers with different geometries is constructed using a near‐field electrostatic printing technique to investigate the regulatory mechanisms of geometry on stem cell function and bone regeneration. The scaffolds precisely mimicked cell dimensions with high porosity and interoperability. Compared with other spatial topography angles, microfibers with a 90° topology can significantly promote the expression of osteogenic gene proteins in bone marrow‐derived mesenchymal stem cells (BMSCs). The effects of different spatial structures on the expression profiles of BMSCs differentiation genes are correlated and validated using microRNA sequencing. Enrichment analysis shows that the 90° microfibers promoted osteogenesis in BMSCs by significantly upregulating miR‐222‐5p/cbfb/Runx2 expression. The ability of the geometric architecture to promote bone regeneration, as assessed using the cranial defect model, demonstrates that the 90° fiber scaffolds significantly promote new bone regeneration and neovascular neural network formation. This study is the first to elucidate the relationship between angular geometry and cellular gene expression, contributing significantly to the understanding of how geometric architecture can promote stem cell differentiation, proliferation, and function for structural bone regeneration. A series of microfibers with different geometries are constructed to investigate the regulatory mechanisms of geometry on stem cell function and bone regeneration. The microfiber patterns with a 90° topology can efficiently promote the expression of osteogenic gene proteins of mesenchymal stem cells through the upregulation of the miR‐222‐5p/cbfb/Runx2 axis, accelerating new bone regeneration and neurovascular networks formation.
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影响因子：
14
作者：
Kang, Yunqing;Kim, Sungvvoo;Bishop, Julius;Khademhosseini, Ali;Yang, Yunzhi
通讯作者：
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影响因子：
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作者：
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5.2
作者：
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影响因子：
64.8
作者：
Kusumbe AP;Ramasamy SK;Adams RH
通讯作者：
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