The use of Loop-mediated Isothermal Amplification (LAMP) to detect the re-emerging Human African Trypanosomiasis (HAT) in the Luangwa and Zambezi valleys.
The use of Loop-mediated Isothermal Amplification (LAMP) to detect the re-emerging Human African Trypanosomiasis (HAT) in the Luangwa and Zambezi valleys.
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DOI：
10.1186/1756-3305-5-282
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发表时间：
2012-12-04
影响因子：
3.2
通讯作者：
Sugimoto C
中科院分区：
文献类型：
--
作者：
Namangala B;Hachaambwa L;Kajino K;Mweene AS;Hayashida K;Simuunza M;Simukoko H;Choongo K;Chansa P;Lakhi S;Moonga L;Chota A;Ndebe J;Nsakashalo-Senkwe M;Chizema E;Kasonka L;Sugimoto C
环介导等温扩增（LAMP）是一种新型的策略，可以在等温条件下高灵敏度、快速地扩增DNA。在本研究中，使用分别来自赞比亚卢安瓜和赞比西河谷的四名男性患者的临床样本评估了重复插入移动的元件（RIME）-LAMP和人血清耐药相关基因（SRA）-LAMP检测的性能。和津巴布韦。本初步通讯中报告的病例均首先通过显微镜诊断，通过被动监测，并通过RIME-LAMP和SRA-LAMP证实。观察到显微镜检查和LAMP之间的良好相关性，有助于患者的分期和成功治疗。RIME-LAMP和SRA-LAMP在所有情况下都能很好地互补。RIME-LAMP和SRA-LAMP都能够检测到患者血液和CSF中的布氏罗得西亚锥虫DNA，因此证实了寄生虫血症患者的HAT。我们的研究表明，LAMP技术是一个潜在的工具，HAT的诊断，分期，并可能是有用的治疗决策。然而，由于本研究中使用的样本量有限，因此无法得出具有统计学意义的结论。因此，必须进行详细的研究，以进一步评估LAMP作为HAT床边诊断试验的潜力。
Loop-mediated isothermal amplification (LAMP) is a novel strategy which amplifies DNA with high sensitivity and rapidity under isothermal conditions. In the present study, the performance of the repetitive insertion mobile element (RIME)-LAMP and human serum resistance-associated gene (SRA)-LAMP assays were evaluated using clinical specimens obtained from four male patients from Luangwa and Zambezi valleys in Zambia and Zimbabwe, respectively. The cases reported in this preliminary communication were all first diagnosed by microscopy, through passive surveillance, and confirmed by both RIME-LAMP and SRA-LAMP. A good correlation between microscopy and LAMP was observed and contributed to staging and successful treatment of patient. RIME-LAMP and SRA-LAMP complimented each other well in all the cases. Both RIME-LAMP and SRA-LAMP were able to detect Trypanosoma brucei rhodesiense DNA in patient blood and CSF and hence confirmed HAT in the parasitaemic patients. Our study indicates that the LAMP technique is a potential tool for HAT diagnosis, staging and may be useful for making therapeutic decisions. However, no statistically significant conclusion may be drawn due to the limited sample size used in the present study. It is thus imperative to conduct a detailed study to further evaluate the potential of LAMP as a bedside diagnostic test for HAT.
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影响因子：
3.3
作者：
Odiit, M;Coleman, PG;Woolhouse, MEJ
通讯作者：
Woolhouse, MEJ
影响因子：
3.8
作者：
Deborggraeve, Stijn;Lejon, Veerle;Buscher, Philippe
通讯作者：
Buscher, Philippe
影响因子：
2.7
作者：
Blum, J;Schmid, C;Burri, C
通讯作者：
Burri, C
DOI：
10.1016/j.trstmh.2010.12.002
发表时间：
2011-03-01
影响因子：
2.2
作者：
Mwanakasale, Victor;Songolo, Peter
通讯作者：
Songolo, Peter
影响因子：
11.2
作者：
Kennedy, Peter G. E.
通讯作者：
Kennedy, Peter G. E.