Saliva is more sensitive than nasopharyngeal or nasal swabs for diagnosis of asymptomatic and mild COVID-19 infection.
Saliva is more sensitive than nasopharyngeal or nasal swabs for diagnosis of asymptomatic and mild COVID-19 infection.
复制标题
唾液比鼻咽或鼻拭子更敏感，用于诊断无症状和轻度COVID-19的感染。
DOI：
10.1038/s41598-021-82787-z
复制
发表时间：
2021-02-04
影响因子：
4.6
通讯作者：
Hsu LY
中科院分区：
文献类型：
--
作者：
Teo AKJ;Choudhury Y;Tan IB;Cher CY;Chew SH;Wan ZY;Cheng LTE;Oon LLE;Tan MH;Chan KS;Hsu LY
我们的目的是在新加坡的一个大型移民工人队列中测试鼻咽唾液和自我给药鼻（SN）拭子与鼻咽（NP）拭子用于COVID-19检测的敏感性。我们还测试了下一代测序（NGS）在COVID-19诊断中的实用性。从患有急性呼吸道感染的受试者、其无症状室友以及2020年6月在社区护理机构接受隔离的既往确诊病例中采集了唾液、NP和SN拭子。所有样本均采用RT-PCR进行检测。对30份样本进行了基于SARS-CoV-2扩增子的NGS和系统发育分析。我们招募了200名受试者，其中91人和46人分别接受了两次和三次测试。唾液、NP和SN的阳性率分别为62.0%、44.5%和37.7%。在所有样本类型的感染早期，循环阈值（Ct）值均较低。唾液检测阳性率高于NP和SN拭子。我们发现NGS的病毒基因组覆盖率与SARS-CoV-2的Ct值之间存在很强的相关性。系统发育分析揭示了已知在新加坡流行的进化枝O和谱系B.6。我们发现唾液是诊断COVID-19的敏感且可行的样本。
We aimed to test the sensitivity of naso-oropharyngeal saliva and self-administered nasal (SN) swab compared to nasopharyngeal (NP) swab for COVID-19 testing in a large cohort of migrant workers in Singapore. We also tested the utility of next-generation sequencing (NGS) for diagnosis of COVID-19. Saliva, NP and SN swabs were collected from subjects who presented with acute respiratory infection, their asymptomatic roommates, and prior confirmed cases who were undergoing isolation at a community care facility in June 2020. All samples were tested using RT-PCR. SARS-CoV-2 amplicon-based NGS with phylogenetic analysis was done for 30 samples. We recruited 200 subjects, of which 91 and 46 were tested twice and thrice respectively. In total, 62.0%, 44.5%, and 37.7% of saliva, NP and SN samples were positive. Cycle threshold (Ct) values were lower during the earlier period of infection across all sample types. The percentage of test-positive saliva was higher than NP and SN swabs. We found a strong correlation between viral genome coverage by NGS and Ct values for SARS-CoV-2. Phylogenetic analyses revealed Clade O and lineage B.6 known to be circulating in Singapore. We found saliva to be a sensitive and viable sample for COVID-19 diagnosis.
登录
查看更多内容
影响因子：
3.9
作者：
Czumbel, Laszlo Mark;Kiss, Szabolcs;Varga, Gabor
通讯作者：
Varga, Gabor
影响因子：
120.7
作者：
Young, Barnaby Edward;Ong, Sean Wei Xiang;Lye, David Chien
通讯作者：
Lye, David Chien
影响因子：
11.8
作者：
Jamal, Alainna J.;Mozafarihashjin, Mohammad;Mubareka, Samira
通讯作者：
Mubareka, Samira
2020 年 1 月至 3 月中国浙江省 SARS-CoV-2 感染患者的病毒载量动态和疾病严重程度：回顾性队列研究
影响因子：
105.7
作者：
Zheng, Shufa;Fan, Jian;Liang, Tingbo
通讯作者：
Liang, Tingbo
影响因子：
56.3
作者：
To, Kelvin Kai-Wang;Tsang, Owen Tak-Yin;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung