Curli synthesis and biofilm formation in enteric bacteria are controlled by a dynamic small RNA module made up of a pseudoknot assisted by an RNA chaperone.
Curli synthesis and biofilm formation in enteric bacteria are controlled by a dynamic small RNA module made up of a pseudoknot assisted by an RNA chaperone.
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肠道细菌中的Curli合成和生物膜形成由由RNA伴侣辅助的伪单元组成的动态小RNA模块控制。
DOI：
10.1093/nar/gku098
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发表时间：
2014-04
影响因子：
14.9
通讯作者：
Felden B
中科院分区：
文献类型：
--
作者：
Bordeau V;Felden B
RydC假结是一个由Hfq辅助的动态调控模块。我们报告说，RydC减少卷曲特异性基因D转录因子的表达所需的粘附和生物膜的生产肠杆菌。在卷曲形成过程中，当缺乏内源性RydC水平时，csgD信使RNA（mRNA）合成增加。在大肠杆菌和沙门氏菌中，RydC表达的刺激也通过损害curli合成来减少生物膜形成。在生长早期诱导RydC降低CsgA、-B和-D蛋白和mRNA水平。RydC的5′-结构域与csgD mRNA翻译起始信号相互作用以阻止起始。翻译抑制通过反义机制发生，通过配对阻断翻译起始信号，并且该机制由Hfq促进。虽然Hfq在没有小RNA（sRNA）的情况下抑制csgD mRNA翻译，但它与RydC形成三元复合物，并促进假结解折叠以与csgD mRNA翻译起始信号相互作用。RydC作用意味着Hfq辅助的解折叠和mRNA重排，但是一旦假结被破坏，Hfq就不需要调节了。RydC是负控制CsgD合成的第六种sRNA。Hfq诱导这六种sRNA靶向的mRNA结构域的结构变化。我们所描述的是一个巧妙的过程，其中假结开放是精心策划的伴侣，让RNA控制基因表达。
RydC pseudoknot aided by Hfq is a dynamic regulatory module. We report that RydC reduces expression of curli-specific gene D transcription factor required for adhesion and biofilm production in enterobacteria. During curli formation, csgD messenger RNA (mRNA) synthesis increases when endogenous levels of RydC are lacking. In Escherichia coli and Salmonella enterica, stimulation of RydC expression also reduces biofilm formation by impairing curli synthesis. Inducing RydC early on in growth lowers CsgA, -B and -D protein and mRNA levels. RydC’s 5′-domain interacts with csgD mRNA translation initiation signals to prevent initiation. Translation inhibition occurs by an antisense mechanism, blocking the translation initiation signals through pairing, and that mechanism is facilitated by Hfq. Although Hfq represses csgD mRNA translation without a small RNA (sRNA), it forms a ternary complex with RydC and facilitates pseudoknot unfolding to interact with the csgD mRNA translation initiation signals. RydC action implies Hfq-assisted unfolding and mRNA rearrangements, but once the pseudoknot is disrupted, Hfq is unnecessary for regulation. RydC is the sixth sRNA that negatively controls CsgD synthesis. Hfq induces structural changes in the mRNA domains targeted by these six sRNAs. What we describe is an ingenious process whereby pseudoknot opening is orchestrated by a chaperone to allow RNA control of gene expression.
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影响因子：
3.6
作者：
Jorgensen, Mikkel Girke;Nielsen, Jesper S.;Valentin-Hansen, Poul
通讯作者：
Valentin-Hansen, Poul
影响因子：
3.6
作者：
Mika F;Busse S;Possling A;Berkholz J;Tschowri N;Sommerfeldt N;Pruteanu M;Hengge R
通讯作者：
Hengge R
影响因子：
3.2
作者：
Collinson, SK;Clouthier, SC;Kay, WW
通讯作者：
Kay, WW
影响因子：
4.5
作者：
Soper, Toby J.;Doxzen, Kevin;Woodson, Sarah A.
通讯作者：
Woodson, Sarah A.
影响因子：
3.6
作者：
ARNQVIST, A;OLSEN, A;NORMARK, S
通讯作者：
NORMARK, S