Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coli.
Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coli.
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DOI：
10.1111/j.1365-2958.2012.08002.x
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发表时间：
2012-04
影响因子：
3.6
通讯作者：
Hengge R
中科院分区：
文献类型：
--
作者：
Mika F;Busse S;Possling A;Berkholz J;Tschowri N;Sommerfeldt N;Pruteanu M;Hengge R
RprA是一种小的调控RNA，已知其对大肠杆菌中σS（RpoS）的翻译有微弱影响。在这里，我们证明，csgD，它编码一个稳定期诱导的生物膜调节剂，以及ydaM，它编码的二鸟苷酸环化酶参与激活csgD转录，是新的负控制RprA目标。如广泛的突变分析所示，RprA与csgD mRNA的5′-非翻译区和翻译起始区的直接结合抑制了翻译并降低了csgD mRNA水平。在ydaM mRNA的情况下，RprA碱基配对直接位于翻译起始密码子的下游。因此，在前馈回路中，RprA可以下调> 30个YdaM/CsgD激活的基因，包括粘附卷曲菌毛的基因。然而，在稳定期早期，当csgD转录被强烈激活时，csgD mRNA的合成超过RprA的合成，这允许CsgD蛋白的积累。当csgD转录被关闭时-例如，稍后在稳定期或在生物膜形成期间-或通过经由Rcs双组分系统进一步激活RprA表达的条件，这种情况被逆转。因此，在mRNA水平上的csgD和RprA的拮抗性调节将细胞包膜应激信号与稳定期和生物膜形成期间的全局基因表达整合。
RprA is a small regulatory RNA known to weakly affect the translation of σS (RpoS) in Escherichia coli. Here we demonstrate that csgD, which encodes a stationary phase-induced biofilm regulator, as well as ydaM, which encodes a diguanylate cyclase involved in activating csgD transcription, are novel negatively controlled RprA targets. As shown by extensive mutational analysis, direct binding of RprA to the 5′-untranslated and translational initiation regions of csgD mRNA inhibits translation and reduces csgD mRNA levels. In the case of ydaM mRNA, RprA base-pairs directly downstream of the translational start codon. In a feedforward loop, RprA can thus downregulate > 30 YdaM/CsgD-activated genes including those for adhesive curli fimbriae. However, during early stationary phase, when csgD transcription is strongly activated, the synthesis of csgD mRNA exceeds that of RprA, which allows the accumulation of CsgD protein. This situation is reversed when csgD transcription is shut off – for instance, later in stationary phase or during biofilm formation – or by conditions that further activate RprA expression via the Rcs two-component system. Thus, antagonistic regulation of csgD and RprA at the mRNA level integrates cell envelope stress signals with global gene expression during stationary phase and biofilm formation.
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影响因子：
14.9
作者：
Afonyushkin, T;Vecerek, B;Moll, I;Bläsi, U;Kaberdin, VR
通讯作者：
Kaberdin, VR
影响因子：
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通讯作者：
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影响因子：
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作者：
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