Experimentally induced active and quiet sleep engage non-overlapping transcriptional programs in Drosophila.
Experimentally induced active and quiet sleep engage non-overlapping transcriptional programs in Drosophila.
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DOI：
10.7554/elife.88198
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发表时间：
2023-11-01
期刊：
影响因子：
7.7
通讯作者：
van Swinderen B
中科院分区：
文献类型：
--
作者：
Anthoney N;Tainton-Heap L;Luong H;Notaras E;Kewin AB;Zhao Q;Perry T;Batterham P;Shaw PJ;van Swinderen B
哺乳动物的睡眠大致可以分为两个不同的生理类别：快速眼动睡眠(REM)和慢波睡眠(SWS)，因此，REM和SWS被认为实现了一系列不同的功能。果蝇黑腹果蝇越来越多地被用作了解睡眠功能的模型，尽管目前尚不清楚果蝇的大脑是否也参与不同类型的睡眠。在这里，我们比较了在果蝇身上实验研究睡眠的两种常用方法：促进睡眠的神经元的光遗传激活和提供促进睡眠的药物加博沙多。我们发现，这些不同的睡眠诱导方法对延长睡眠时间的效果相似，但对大脑活动的影响不同。转录分析显示，药物诱导的深度睡眠(“安静”睡眠)主要下调新陈代谢基因，而光遗传“活跃”睡眠上调一系列与正常清醒功能相关的基因。这表明，光遗传学和药物诱导果蝇的睡眠促进了不同的睡眠特征，这些睡眠涉及不同的基因集来实现各自的功能。
Sleep in mammals can be broadly classified into two different physiological categories: rapid eye movement (REM) sleep and slow-wave sleep (SWS), and accordingly REM and SWS are thought to achieve a different set of functions. The fruit fly Drosophila melanogaster is increasingly being used as a model to understand sleep functions, although it remains unclear if the fly brain also engages in different kinds of sleep as well. Here, we compare two commonly used approaches for studying sleep experimentally in Drosophila: optogenetic activation of sleep-promoting neurons and provision of a sleep-promoting drug, gaboxadol. We find that these different sleep-induction methods have similar effects on increasing sleep duration, but divergent effects on brain activity. Transcriptomic analysis reveals that drug-induced deep sleep (‘quiet’ sleep) mostly downregulates metabolism genes, whereas optogenetic ‘active’ sleep upregulates a wide range of genes relevant to normal waking functions. This suggests that optogenetics and pharmacological induction of sleep in Drosophila promote different features of sleep, which engage different sets of genes to achieve their respective functions.
影响因子：
4.5
作者：
He Q;Du J;Wei L;Zhao Z
通讯作者：
Zhao Z
影响因子：
9.2
作者：
Gong, Naihua N.;Luong, Hang Ngoc Bao;Dang, An H.;Mainwaring, Benjamin;Shields, Emily;Schmeckpeper, Karl;Bonasio, Roberto;Kayser, Matthew S.
通讯作者：
Kayser, Matthew S.