Intrinsic maturation of sleep output neurons regulates sleep ontogeny in Drosophila.
Intrinsic maturation of sleep output neurons regulates sleep ontogeny in Drosophila.
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DOI：
10.1016/j.cub.2022.07.054
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发表时间：
2022-09-26
期刊：
影响因子：
9.2
通讯作者：
Kayser, Matthew S.
中科院分区：
文献类型：
--
作者：
Gong, Naihua N.;Luong, Hang Ngoc Bao;Dang, An H.;Mainwaring, Benjamin;Shields, Emily;Schmeckpeper, Karl;Bonasio, Roberto;Kayser, Matthew S.
一生中睡眠行为的成熟（睡眠个体发生）是一种进化保守的现象。哺乳动物研究表明，除了增加睡眠时间外，与成熟睡眠相比，早期睡眠表现出明显的睡眠状态差异。睡眠输出回路的内在成熟如何促进睡眠个体发生尚不清楚。黑腹果蝇从幼年到成年表现出多方面的睡眠变化。在这里，我们使用非侵入性概率方法来研究幼蝇和成熟果蝇睡眠结构的变化。幼蝇的睡眠增加主要是由于过渡到清醒状态的可能性降低，其特征是处于深度睡眠状态的时间更长。扇形体（dFB）中睡眠促进神经元的功能操作表明，这些神经元在幼年和成熟果蝇中调节睡眠的差异。不同年龄dFB神经元的转录组学分析和随后的RNAi筛选暗示了参与dFB睡眠回路成熟的基因。这些结果表明，睡眠输出神经元的动态转录状态有助于整个生命周期的睡眠变化。所有动物，从人类到苍蝇，年轻时都睡得更多。生命早期睡眠的分子决定因素在很大程度上仍然未知。Gong等人使用非侵入性概率方法定义了果蝇成熟过程中睡眠结构的变化。他们的发现为睡眠个体发生的机制提供了新的见解。
The maturation of sleep behavior across a lifespan (sleep ontogeny) is an evolutionarily conserved phenomenon. Mammalian studies have shown that in addition to increased sleep duration, early life sleep exhibits stark differences compared to mature sleep with regard to sleep states. How intrinsic maturation of sleep output circuits contributes to sleep ontogeny is poorly understood. The fruit fly Drosophila melanogaster exhibits multifaceted changes to sleep from juvenile to mature adulthood. Here, we use a non-invasive probabilistic approach to investigate changes in sleep architecture in juvenile and mature flies. Increased sleep in juvenile flies is driven primarily by a decreased probability of transitioning to wake, and characterized by more time in deeper sleep states. Functional manipulations of sleep-promoting neurons in the dorsal fan-shaped body (dFB) suggest these neurons differentially regulate sleep in juvenile and mature flies. Transcriptomic analysis of dFB neurons at different ages and a subsequent RNAi screen implicate genes involved in dFB sleep circuit maturation. These results reveal that dynamic transcriptional states of sleep output neurons contribute to changes in sleep across the lifespan. All animals, from humans to flies, sleep more when young. The molecular determinants of sleep during early life remain largely unknown. Using non-invasive probabilistic approaches, Gong et al. define changes in sleep architecture that occur with maturation in Drosophila. Their findings provide novel insight into mechanisms of sleep ontogeny.
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影响因子：
13.6
作者：
Cao J;Herman AB;West GB;Poe G;Savage VM
通讯作者：
Savage VM
两种多巴胺能神经元向背扇形身体发出信号，以促进果蝇的清醒。
影响因子：
9.2
作者：
Liu, Qili;Liu, Sha;Kodama, Lay;Driscoll, Maria R.;Wu, Mark N.
通讯作者：
Wu, Mark N.
影响因子：
64.5
作者：
Davie K;Janssens J;Koldere D;De Waegeneer M;Pech U;Kreft Ł;Aibar S;Makhzami S;Christiaens V;Bravo González-Blas C;Poovathingal S;Hulselmans G;Spanier KI;Moerman T;Vanspauwen B;Geurs S;Voet T;Lammertyn J;Thienpont B;Liu S;Konstantinides N;Fiers M;Verstreken P;Aerts S
通讯作者：
Aerts S
DOI：
10.1111/apa.15485
发表时间：
2021-01
期刊：
Acta paediatrica (Oslo, Norway : 1992)
影响因子：
--
作者：
Knoop MS;de Groot ER;Dudink J
通讯作者：
Dudink J
影响因子：
5.6
作者：
Dilley, Leela C.;Vigderman, Abigail;Kayser, Matthew S.
通讯作者：
Kayser, Matthew S.