In vivo calcium imaging and Parkinson’s disease
In vivo calcium imaging and Parkinson’s disease
复制标题
体内钙成像和帕金森病
DOI：
10.1007/s11427-016-0356-6
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Qinyong Ye
中科院分区：
文献类型：
--
作者：
Tianwen Huang;Qinyong Ye
脑功能取决于突触输入和神经回路的模式，其伴随着游离细胞内钙浓度的瞬时变化（Resendez等人，2015）。最近的一个感兴趣的话题一直是照明的神经元回路活动的钙成像在体内。作为一种记录动物脑内行为信号的新生物学工具，在体钙离子成像技术可以根据细胞内钙离子的变化来监测神经元的活动。体内钙成像是一种先进的技术，通过它可以真实的实时观察单个细胞和/或甚至亚细胞区室。因此，它很容易实现单个细胞和亚细胞区室的同时记录。众所周知，大多数去极化电信号依赖于钙内流来激活神经系统中的电压门控通道，然后可以被细胞内钙储存进一步放大，导致神经元中的多种生理反应，例如突触传递、神经元可塑性、神经元发育、神经元迁移和神经突生长。另一方面，钙的瞬时细胞内升高涉及神经元死亡或神经变性（Ding等人，2014）。因此，所有的证据表明，钙信号是强制性的神经元活动。因此，追踪体内钙的动态变化将有助于我们更好地了解神经元功能，甚至大脑功能。然而，实现高质量动态钙图像的关键取决于钙指示剂和成像设备。钙指示剂主要有两类：化学指示剂和遗传编码钙指示剂（GECI）。化学指示剂，包括fura-2、indo-1、fluo-3、fluo-4和钙绿色-1，是可以螯合钙离子的小分子，并用于多种细胞中的细胞溶质钙测量。然而，这些膜渗透形式的荧光钙指示剂只能令人满意地加载细胞培养物或未成熟脑组织切片中的神经元群体。GECI是来源于绿色荧光蛋白（GFP）或其变体（如环状排列的GFP、YFP和CFP）的荧光蛋白，其与肌球蛋白轻链激酶和钙调蛋白（CaM）的M13结构域融合，钙调蛋白能够结合钙。编码GECI的基因容易转移到细胞系中。同时，有可能产生在所有细胞或某些细胞亚型中表达指示剂的转基因动物。由于GCaMP 6在细胞环境中显示出高灵敏度和明亮的基线荧光，因此已被用于树突状细胞的钙成像。
Brain function depends on patterns of synaptic input and neurocircuits, which are accompanied by transient changes in free intracellular calcium concentration (Resendez et al., 2015). A recent topic of interest has been the illumination of neuronal circuit activity by calcium imaging in vivo. As a new biological tool for recording behavioral signals in the animal brain, in vivo calcium imaging is performed to monitor neuronal activity based on intracellular calcium. In vivo calcium imaging is an advanced technique by which individual cells and/or even subcellular compartments can be observed in real time. Thus, it readily enables simultaneous recording of individual cells and subcellular compartments. It is widely known that most depolarizing electrical signals rely on calcium influx to activate voltage-gated channels in the nervous system, which may then be further amplified by intracellular calcium stores, resulting in multiple physiological responses in neurons, such as synaptic transmission , neuronal plasticity, neuronal development, neu-ronal migration, and neurite outgrowth. On the other hand, transient intracellular elevation of calcium is involved in neuronal death or neurodegeneration (Ding et al., 2014). Thus, all of the evidence presented demonstrates that calcium signaling is obligatory for neuronal activities. Therefore , tracing dynamic changes of calcium in vivo will help us better understand neuronal function, and even brain function. However, the key to achieving high-quality dynamic calcium images is dependent on calcium indicators and imaging devices. There are two main classes of calcium indicators: chemical indicators and genetically encoded calcium indicators (GECI). The chemical indicators, including fura-2, indo-1, fluo-3, fluo-4, and calcium green-1, are small molecules that can chelate calcium ions, and are utilized for cytosolic calcium measurements in a wide variety of cells. However, these membrane-permeant forms of fluorometric calcium indicators are only able to satisfactorily load populations of neurons in cell culture or in slices of immature brain tissue. GECI are fluorescent proteins derived from green fluorescent protein (GFP) or its variants (such as circularly per-muted GFP, YFP, and CFP), which fuse with the M13 domain of the myosin light chain kinase and calmodulin (CaM), which is able to bind calcium. The genes encoding for GECI are easily transferred to cell lines. At the same time, it is possible to create a transgenic animal expressing the indicator in all cells or certain cellular subtypes. Because it showed high sensitivity and bright baseline fluo-rescence in a cellular environment, GCaMP6 has been used for calcium imaging of dendritic …
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影响因子：
6.1
作者：
Cui ZQ;Luan GM;Zhou J;Zhai F;Guan YG;Bao M
通讯作者：
Bao M
影响因子：
6.1
作者：
Fang J;Cui LY;Liu MS;Guan YZ;Li XG;Cui B;Ding QY
通讯作者：
Ding QY
影响因子：
6.1
作者：
Fang J;Liu MS;Guan YZ;Cui B;Cui LY
通讯作者：
Cui LY
影响因子：
6.1
作者：
Yi Ding;Cunqing Chang;Lan Xie;Zhi-min Chen;Hua Ai
通讯作者：
Yi Ding;Cunqing Chang;Lan Xie;Zhi-min Chen;Hua Ai
DOI：
10.1073/pnas.1232232100
发表时间：
2003-06-10
影响因子：
11.1
作者：
Stosiek, C;Garaschuk, O;Konnerth, A
通讯作者：
Konnerth, A