CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis.
CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis.
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CENP-F是一种核基质的蛋白质，该核基质在G2晚期聚集在动力学上，并在有丝分裂后迅速降解。
DOI：
10.1083/jcb.130.3.507
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发表时间：
1995-08
影响因子：
7.8
通讯作者：
YEN, TJ
中科院分区：
文献类型：
--
作者：
LIAO, H;WINKFEIN, RJ;MACK, G;RATTNER, JB;YEN, TJ
着丝粒蛋白-F（CENP-F）是最近通过自身免疫血清鉴定的哺乳动物动粒蛋白（Rattner，J. B.，A.拉奥，M。J. Fritzler，D. W.瓦伦西亚和T. J. Yen。细胞动力。细胞骨架。26：214-226）。我们在这里报告的CENP-F的人cDNA序列，沿着其在HeLa细胞周期的不同阶段的表达和定位模式。CENP-F是核基质的蛋白质，其在细胞周期期间逐渐积累，直到其在G2和M期细胞中达到峰值水平，并在有丝分裂完成时迅速降解。CENP-F首先在G2晚期在前动粒复合体处检测到，并且作为对应于前期所有着丝粒的成对病灶而清楚地检测到。在有丝分裂过程中，CENP-F与从前中期到后期早期的动粒相关，然后在整个后期的剩余部分在纺锤体中间区检测到。到末期，CENP-F集中在中间体两侧的细胞内桥内。367 kD CENP-F蛋白的预测结构由两个1,600个氨基酸长的螺旋结构域组成，它们位于中心柔性核心的侧面。一个假定的P环核苷酸结合位点（ADIPTGKT）位于球状羧基末端。从我们的序列研究推断的结构特征和CENP-F的空间和时间分布在我们的细胞学和生物化学研究表明，它可能在几个有丝分裂事件中发挥作用。
Centromere protein-F (CENP-F) is mammalian kinetochore protein that was recently identified by an autoimmune serum (Rattner, J. B., A. Rao, M. J. Fritzler, D. W. Valencia, and T. J. Yen. Cell Motil. Cytoskeleton. 26:214-226). We report here the human cDNA sequence of CENP-F, along with its expression and localization patterns at different stages of the HeLa cell cycle. CENP-F is protein of the nuclear matrix that gradually accumulates during the cell cycle until it reaches peak levels in G2 and M phase cells and is rapidly degraded upon completion of mitosis. CENP-F is first detected at the prekinetochore complex during late G2, and is clearly detectable as paired foci that correspond to all the centromeres by prophase. During mitosis, CENP-F is associated with kinetochores from prometaphase until early anaphase and is then detected at the spindle midzone throughout the remainder of anaphase. By telophase, CENP-F is concentrated within the intracellular bridge at either side of the mid-body. The predicted structure of the 367-kD CENP-F protein consists of two 1,600-amino acid-long coil domains that flank a central flexible core. A putative P-loop nucleotide binding site (ADIPTGKT) is located within the globular carboxy terminus. The structural features deduced from our sequence studies and the spatial and temperal distribution of CENP-F revealed in our cytological and biochemical studies suggest that it may play a role in several mitotic events.
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影响因子：
7.8
作者：
GOH, PY;KILMARTIN, JV
通讯作者：
KILMARTIN, JV
DOI：
10.1073/pnas.83.4.907
发表时间：
1986-02-01
影响因子：
11.1
作者：
FRY, DC;KUBY, SA;MILDVAN, AS
通讯作者：
MILDVAN, AS
影响因子：
64.8
作者：
HYMAN, AA;MIDDLETON, K;CARBON, J
通讯作者：
CARBON, J
DOI：
10.1083/jcb.127.2.303
发表时间：
1994-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Saitoh N;Goldberg IG;Wood ER;Earnshaw WC
通讯作者：
Earnshaw WC
DOI：
10.1073/pnas.91.15.7212
发表时间：
1994-07-19
影响因子：
11.1
作者：
MIDDLETON, K;CARBON, J
通讯作者：
CARBON, J