Extended-spectrum ß-lactamase-producing Escherichia coli among humans, chickens and poultry environments in Abuja, Nigeria.
Extended-spectrum ß-lactamase-producing Escherichia coli among humans, chickens and poultry environments in Abuja, Nigeria.
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DOI：
10.1186/s42522-020-00014-7
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发表时间：
2020
影响因子：
4.9
通讯作者：
Thakur S
中科院分区：
文献类型：
--
作者：
Aworh MK;Kwaga J;Okolocha E;Harden L;Hull D;Hendriksen RS;Thakur S
在全球范围内，鸡被认为是向人类传播抗微生物药物耐药性基因的储存库。在尼日利亚，很容易获得用于家禽生产的抗菌药物，无论是用于预防还是治疗目的。广谱β -内酰胺酶产生大肠杆菌（ESBL-EC）由于其人畜共患的潜力而传播给人类。在农场或市场上与鸡密切接触的人面临的风险更大。本研究的目的是调查尼日利亚阿布贾家禽工人、鸡和家禽环境中esble - ec的流行情况和人畜共患传播。我们在阿布贾选定的农场/鸡肉市场对工人、鸡和家禽环境进行了横断面研究。从2018年12月至2019年4月，从表面健康的工人、鸡和农场/市场环境中收集粪便、粪便和环境样本。使用安装在智能手机上的开放数据工具包（ODK）以电子方式收集数据。采用肉汤微量稀释法测定对14种抗菌素的耐药性。我们对分离株进行了表型和基因型鉴定。通过计算频率、比例和spearman相关系数（ρ）对数据进行分析。在429份样本中，大肠杆菌阳性的占26.8% （n = 115）。115株大肠杆菌分离株中，32.2% （n = 37）经表型鉴定为ESBL产生菌。禽工（37.8%,n = 14）和鸡（37.8%,n = 14）中esble - ec患病率最高，其次是环境（24.3%,n = 9）。人和鸡分离株对所测抗菌素的多药耐药模式相似，呈正相关（ρ = 0.91）。在ESBL生产者中，我们观察到blaCTX-M基因的传播（10.8%,n = 4）。8.1% （n = 3）的分离株中同时存在blaCTX-M-15和blactm -1基因，其中66.7% （n = 2）为鸡场分离株，1例人源分离株为鸡市场分离株。esble - ec分离株在阿布贾明显健康的个体、鸡和家禽养殖场/市场环境中普遍存在。重要的是要教育卫生保健工作者，与家禽接触的人是粪便携带esble - ec的高危人群，因此对一般人群造成较高的抗微生物药物耐药性传播风险。
Globally, chicken is known to be a reservoir for the spread of antimicrobial resistance genes to humans. In Nigeria, antimicrobial drugs are readily accessible for use in poultry production, either for preventive or therapeutic purposes. Extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) are transmissible to humans because of their zoonotic potentials. People working very closely with chickens either on farms or markets are at greater risk. The aim of this study was to investigate the prevalence and zoonotic transmission of ESBL-EC among poultry-workers, chickens, and poultry environments in Abuja, Nigeria. We conducted a cross-sectional study among workers, chickens and poultry environment in selected farms/chicken markets in Abuja. Stool, faecal, and environmental samples were collected from apparently healthy workers, chickens, and farm/market environments from December 2018 to April 2019. Data were collected electronically using an open data kit (ODK) installed on a Smartphone. Antimicrobial resistance was determined using broth micro-dilution methods against a panel of 14 antimicrobial agents. We carried out the phenotypic and genotypic characterization of the isolates. Data were analyzed by computing frequencies, proportions and spearman’s correlation (ρ). Of 429 samples, 26.8% (n = 115) were positive for Escherichia coli (E. coli). Of the 115 E. coli isolates, 32.2% (n = 37) were confirmed ESBL producers by phenotypic characterization. Prevalence of ESBL-EC was highest among both poultry-workers (37.8%; n = 14) and chickens (37.8%; n = 14) followed by the environment (24.3%; n = 9). Both human and chicken isolates showed similar patterns of multidrug resistance to tested antimicrobials with a positive correlation (ρ = 0.91). Among ESBL producers, we observed the dissemination of blaCTX-M (10.8%; n = 4) genes. The coexistence of blaCTX-M-15 and blaTEM-1 genes was observed in 8.1% (n = 3) of the isolates, out of which (66.7%; n = 2) were chicken isolates from the farm, while a single human isolate was from the chicken market. ESBL-EC isolates were prevalent amongst apparently healthy individuals, chickens and the poultry farm/market environment in Abuja. It is important to educate healthcare workers that people in proximity with poultry are a high-risk group for faecal carriage of ESBL-EC, hence pose a higher risk to the general population for the spread of antimicrobial resistance.
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影响因子：
9.4
作者：
Veenemans, J.;Overdevest, I. T.;Kluytmans, J. A. J. W.
通讯作者：
Kluytmans, J. A. J. W.
影响因子：
3.7
作者：
Aworh, Mabel Kamweli;Kwaga, Jacob;Thakur, Siddhartha
通讯作者：
Thakur, Siddhartha
影响因子：
5.2
作者：
Falgenhauer, Linda;Imirzalioglu, Can;Eibach, Daniel
通讯作者：
Eibach, Daniel
影响因子：
9.4
作者：
Larsen, Mette V.;Cosentino, Salvatore;Lund, Ole
通讯作者：
Lund, Ole
影响因子：
4.4
作者：
Gomi, Ryota;Matsuda, Tomonari;Yoneda, Minoru
通讯作者：
Yoneda, Minoru