Identification and characterization of a novel multi-stress responsive gene in Arabidopsis.
Identification and characterization of a novel multi-stress responsive gene in Arabidopsis.
复制标题
拟南芥中新型多应力响应基因的识别和表征。
DOI：
10.1371/journal.pone.0244030
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Asim N
中科院分区：
文献类型：
--
作者：
Tawab F;Munir I;Nasim Z;Khan MS;Tawab S;Nasim A;Iqbal A;Ahmad MA;Ali W;Munir R;Munir M;Asim N
非生物胁迫，特别是盐分、干旱和高温，导致作物产量显著下降。在本研究中，我们发现AT4G18280是一个富含甘氨酸的细胞壁蛋白样蛋白(以下简称GRPL1)，是一个潜在的多胁迫响应基因。公共转录组数据分析和GUS分析表明，GRPL1在干旱、盐分和高温胁迫下均有较强的诱导作用。高表达GRPL1-3HA的转基因植株在盐胁迫下表现出较高的发芽率、根伸长和成活率。此外，35S：：GRPL1-3HA转基因株系在干旱和高温胁迫下也表现出较高的存活率。GRPL1在不同的生长和胁迫条件下表现出与脱落酸(ABA)途径基因相似的表达模式，这表明GRPL1可能作用于ABA途径，而ABA缺失突变体(aba2-1)在干旱胁迫下不能诱导GRPL1的表达。综上所述，我们的数据表明GRPL1是一个潜在的作用于ABA下游的多胁迫反应基因。
Abiotic stresses especially salinity, drought and high temperature result in considerable reduction of crop productivity. In this study, we identified AT4G18280 annotated as a glycine-rich cell wall protein-like (hereafter refer to as GRPL1) protein as a potential multistress-responsive gene. Analysis of public transcriptome data and GUS assay of pGRPL1::GUS showed a strong induction of GRPL1 under drought, salinity and heat stresses. Transgenic plants overexpressing GRPL1-3HA showed significantly higher germination, root elongation and survival rate under salt stress. Moreover, the 35S::GRPL1-3HA transgenic lines also showed higher survival rates under drought and heat stresses. GRPL1 showed similar expression patterns with Abscisic acid (ABA)-pathway genes under different growth and stress conditions, suggesting a possibility that GRPL1 might act in the ABA pathway that is further supported by the inability of ABA-deficient mutant (aba2-1) to induce GRPL1 under drought stress. Taken together, our data presents GRPL1 as a potential multi-stress responsive gene working downstream of ABA.
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bHLH122 对于拟南芥的干旱和渗透胁迫抵抗以及 ABA 分解代谢的抑制非常重要
影响因子：
9.4
作者：
Liu, Wenwen;Tai, Huanhuan;Li, Wen-Xue
通讯作者：
Li, Wen-Xue
影响因子：
5.6
作者：
Jiang K;Moe-Lange J;Hennet L;Feldman LJ
通讯作者：
Feldman LJ
DOI：
10.1126/science.1186834
发表时间：
2010-02-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fedoroff NV;Battisti DS;Beachy RN;Cooper PJ;Fischhoff DA;Hodges CN;Knauf VC;Lobell D;Mazur BJ;Molden D;Reynolds MP;Ronald PC;Rosegrant MW;Sanchez PA;Vonshak A;Zhu JK
通讯作者：
Zhu JK
影响因子：
6.4
作者：
Hernández, JA;Almansa, MS
通讯作者：
Almansa, MS
影响因子：
2.9
作者：
Kim, Sang-Gyu;Park, Chung-Mo
通讯作者：
Park, Chung-Mo