Molecular Screening of Blood Donors for Babesia in Tyrol, Austria.
Molecular Screening of Blood Donors for Babesia in Tyrol, Austria.
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DOI：
10.1159/000528793
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发表时间：
2023-08
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影响因子：
--
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中科院分区：
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巴贝虫是一种蜱传播的红细胞内寄生虫，在全球普遍存在，但研究不足。几种巴氏杆菌已被证明是输血传播的。在蒂罗尔州（奥地利西部）的献血者、动物和蜱中报告了巴贝虫病，并描述了人类巴贝虫病的区域病例。我们试图描述巴氏杆菌对当地血液供应的风险。对在奥地利蒂罗尔州（2021年5月27日至10月4日）参加区域移动的采血活动的献血者进行了前瞻性分子检测。使用cobas<sup>®</sup>巴氏杆菌测定法（Roche Molecular Systems，Inc.）批准用于献血者筛选的商业PCR测定能够检测引起人类巴贝虫病的4种主要物种（即，B。microti，B. divergens，B. duncani和B. venatorum）。开发了管理初始PCR反应性样本的验证性算法，以及供体和产品管理程序。共招募和筛选了7，972名供体; 4，311名（54.1%）为男性，中位年龄为47岁（IQR = 34-55）。未检测到巴氏杆菌阳性病例，相应的总体患病率为0.00%（95% CI：0.00%，0.05%）。研究结果表明，即使在蜱虫暴露高峰期的几个月里，居住在蒂罗尔州的奥地利献血者中的巴伯利什流行率也很低。尽管由于样本量有限，无法得出该人群中不存在巴贝虫的结论，但研究结果表明，输血传播的巴贝虫病的区域风险较低。
Babesia is a tick-borne intraerythrocytic parasite that is globally ubiquitous, yet understudied. Several species of Babesia have been shown to be transfusion-transmissible. Babesia has been reported in blood donors, animals, and ticks in the Tyrol (Western Austria), and regional cases of human babesiosis have been described. We sought to characterize the risk of Babesia to the local blood supply. Prospective molecular testing was performed on blood donors who presented to regional, mobile blood collection drives in the Tyrol, Austria (27 May to October 4, 2021). Testing was conducted using the cobas<sup>®</sup> Babesia assay (Roche Molecular Systems, Inc.), a commercial PCR assay approved for blood donor screening that is capable of detecting the 4 primary species causing human babesiosis (i.e., B. microti, B. divergens, B. duncani, and B. venatorum). A confirmatory algorithm to manage initial PCR-reactive samples was developed, as were procedures for donor and product management. A total of 7,972 donors were enrolled and screened; 4,311 (54.1%) were male, with a median age of 47 years (IQR = 34–55). No positive cases of Babesia were detected, corresponding with an overall prevalence of 0.00% (95% CI: 0.00%, 0.05%). The findings suggest that the prevalence of Babesia is low in Austrian blood donors residing in the Tyrol, even during months of peak tick exposure. Although one cannot conclude the absence of Babesia in this population given the limited sample size, the findings suggest that the regional risk of transfusion-transmitted babesiosis is low.
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影响因子：
2.6
作者：
Meer-Scherrer, L;Adelson, M;Tilton, R
通讯作者：
Tilton, R
影响因子：
4.4
作者：
Vannier, Edouard G.;Diuk-Wasser, Maria A.;Krause, Peter J.
通讯作者：
Krause, Peter J.
DOI：
10.1007/s10096-007-0333-1
发表时间：
2007-08-01
影响因子：
4.5
作者：
Hildebrandt, A.;Hunfeld, K.-P.;Straube, E.
通讯作者：
Straube, E.
影响因子：
158.5
作者：
Moritz, Erin D.;Winton, Colleen S.;Stramer, Susan L.
通讯作者：
Stramer, Susan L.
影响因子：
4.2
作者：
Menis, Mikhail;Whitaker, Barbee, I;Forshee, Richard A.
通讯作者：
Forshee, Richard A.