Multiparametric image analysis of lung-branching morphogenesis.
Multiparametric image analysis of lung-branching morphogenesis.
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DOI：
10.1002/dvdy.23961
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发表时间：
2013-06
影响因子：
2.5
通讯作者：
Niswander, Lee
中科院分区：
文献类型：
--
作者：
Schnatwinkel, Carsten;Niswander, Lee
Lung branching morphogenesis is a fundamental developmental process, yet the cellular dynamics that occur during lung development and the molecular mechanisms underlying recent postulated branching modes are poorly understood. Here, we implemented a time-lapse video microscopy method to study the cellular behavior and molecular mechanisms of planar bifurcation and domain branching in lung explant- and organotypic cultures. Our analysis revealed morphologically distinct stages that are shaped at least in part by a combination of localized and orientated cell divisions and by local mechanical forces. We also identified myosin light-chain kinase as an important regulator of bud bifurcation, but not domain branching in lung explants. This live imaging approach provides a method to study cellular behavior during lung branching morphogenesis and suggests the importance of a mechanism primarily based on oriented cell proliferation and mechanical forces in forming and shaping the developing lung airways.
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