Standardisation of disk diffusion results for antibiotic susceptibility testing using the sirscan automated zone reader.
Standardisation of disk diffusion results for antibiotic susceptibility testing using the sirscan automated zone reader.
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DOI：
10.1186/1471-2180-13-225
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发表时间：
2013-10-08
期刊：
影响因子：
4.2
通讯作者：
Böttger EC
中科院分区：
文献类型：
--
作者：
Hombach M;Zbinden R;Böttger EC
纸片扩散读数的标准化可以提高抗生素敏感性试验(AST)的重复性和准确性。这项研究评估了使用“Sirscan Automatic”区带阅读器(I2a，Perols Cedex，法国)的自动抑制区带读数的准确性、重复性和精密度。在第一步中，我们将Sirscan结果与手动卡尺测量结果进行了比较，以确定可比性和准确性。根据制造商的建议，在屏幕上检查和调整SirScan读数。对代表临床实验室常规分离的广谱微生物的100个临床细菌分离株进行了测试，并比较了区带直径值和根据EUCAST指南的解释。在第二步中，我们分析了全自动区域读数是否可以减少直径测量的标准偏差，从而提高圆盘扩散法的重复性和精度。对金黄色葡萄球菌ATCC 29213、大肠杆菌ATCC 25922和铜绿假单胞菌ATCC 27853(EUCAST质量控制菌株)的19个重复独立的抑制区进行了手动测量、屏幕调整的SIRSCAN测量和全自动SIRSCAN读数的标准偏差比较。屏幕上调整后的SirScan和卡尺测量显示出很高的可比性。比较两种读数方法的结果，未发现显著差异。与调整后的Sirscan读数和手动方法相比，全自动SirScan测量的抑制区直径的标准偏差显著降低，从而使自动读数的重复性和精确度更好。我们的结果表明，全自动区段读数可以通过减小标准差来进一步提高AST的标准化程度，从而提高抑制区段直径结果的精度。
Standardisation of disk diffusion readings could improve reproducibility and accuracy of antibiotic susceptibility testing (AST). This study evaluated accuracy, reproducibility, and precision of automated inhibition zone reading using the “Sirscan automatic” zone reader (i2a, Perols Cedex, France). In a first step we compared Sirscan results with manual calliper measurements for comparability and accuracy. Sirscan readings were checked and adjusted on-screen as recommended by the manufacturer. One hundred clinical bacterial isolates representing a broad spectrum of organisms routinely isolated in a clinical laboratory were tested, and zone diameter values and interpretation according to EUCAST guidelines were compared. In a second step we analysed, whether fully automated zone reading can decrease standard deviation of diameter measurements and, thus, improve reproducibility and precision of the disk diffusion method. Standard deviations of manual measurements, on-screen adjusted Sirscan measurements, and fully automated Sirscan readings were compared for 19 repeat independent measurements of inhibition zones of S. aureus ATCC 29213, E. coli ATCC 25922, and P. aeruginosa ATCC 27853 (EUCAST quality control strains). On-screen adjusted Sirscan and calliper measurements displayed high comparability. No significant differences were detected comparing the results of both reading methods. Standard deviations of inhibition zone diameters were significantly lower for fully automated Sirscan measurements compared with both adjusted Sirscan readings and the manual method, resulting in better reproducibility and precision of the automated readings. Our results indicate that fully automated zone reading can further improve standardisation of AST by decreasing standard deviation and, thus, improve precision of inhibition zone diameter results.
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