Isolation of Temperature-Sensitive Mutants of Arabidopsis thaliana That Are Defective in the Redifferentiation of Shoots
Isolation of Temperature-Sensitive Mutants of Arabidopsis thaliana That Are Defective in the Redifferentiation of Shoots
复制标题
芽再分化缺陷的拟南芥温度敏感突变体的分离
作者：
Izumi Yasutani;Shoichi Ozawa;Toshiaki Nishida;Munetaka Sugiyama;Atsushi Komamine
筛选出3个在芽再分化过程中存在缺陷的拟南芥温度敏感突变体，作为器官发生研究的工具。从每株M_2植株上分别收获M_3代种子，构建M_3代品系。对2700个M3品系的根外植体在22℃(允许温度)和27℃(限制温度)下的芽再分化进行了比较研究，鉴定出7个温度敏感突变系。对7个突变株系中的3个进行的遗传测试表明，这3个突变系对温度敏感的再分化是由SRD_1、SRD_2和SRD_3三个不同基因的单核隐性突变引起的。在限制温度下培养的SRD突变体的根外植体的形态表明，这些SRD基因的产物在芽的再分化的不同阶段发挥作用。
Three temperature-sensitive mutants of Arabidopsis thaliana that were defective in the redifferentiation of shoots were isolated as tools for the study of organogenesis. M3 lines were constructed by harvesting M3 seeds separately from each M2 plant. Comparative examination of shoot redifferentiation in root explants of 2700 M3 lines at 22[deg]C (permissive temperature) and at 27[deg]C (restrictive temperature) led to the identification of seven temperature-sensitive mutant lines. Genetic tests of three of the seven mutant lines indicated that temperature-sensitive redifferentiation of shoots in these three lines resulted from single, nuclear, recessive mutations in three different genes, designated SRD1, SRD2, and SRD3. The morphology of root explants of srd mutants cultured at the restrictive temperature suggests that the products of these SRD genes function at different stages of the redifferentiation of shoots.
温度敏感的胡萝卜变体会损害体细胞胚胎发生。
影响因子：
2.7
作者：
Breton,AM;Sung,ZR
通讯作者：
Sung,ZR