In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli.
In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli.
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DOI：
10.1111/j.1365-2958.2009.06776.x
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发表时间：
2009-08
影响因子：
3.6
通讯作者：
Buck M
中科院分区：
文献类型：
--
作者：
Engl C;Jovanovic G;Lloyd LJ;Murray H;Spitaler M;Ying L;Errington J;Buck M
革兰氏阴性菌中的噬菌体休克蛋白（Psp）反应抵消膜应激。大肠杆菌中PspF调节子（pspABCDE和pspG）的转录在耗散质子动力（pmf）的应力下诱导。使用GFP融合，我们已经可视化的亚细胞定位的PspA（Psp的负调节器和效应器）和PspG（Psp的效应器）。先前已经提出PspA均匀地科茨涂覆细胞的内膜。我们现在证明，PspA（和PspG）不是均匀地覆盖整个细胞，而是高度组织成似乎是不同的功能类别（细胞极和细胞侧壁的复合物）。实时观察揭示了侧向PspA和PspG复合物是高度移动的，但在缺乏MreB的细胞中不存在。在没有MreB细胞骨架的情况下，仍然观察到Psp反应的诱导，但这些细胞在应激条件下不能维持pmf。因此，这两个空间亚种在动力学和功能上似乎是不同的，极性集群与感觉功能和移动的复合物与维持pmf相关。
The phage shock protein (Psp) response in Gram-negative bacteria counteracts membrane stress. Transcription of the PspF regulon (pspABCDE and pspG) in Escherichia coli is induced upon stresses that dissipate the proton motive force (pmf). Using GFP fusions we have visualized the subcellular localizations of PspA (a negative regulator and effector of Psp) and PspG (an effector of Psp). It has previously been proposed that PspA evenly coates the inner membrane of the cell. We now demonstrate that instead of uniformly covering the entire cell, PspA (and PspG) is highly organized into what appear to be distinct functional classes (complexes at the cell pole and the lateral cell wall). Real-time observations revealed lateral PspA and PspG complexes are highly mobile, but absent in cells lacking MreB. Without the MreB cytoskeleton, induction of the Psp response is still observed, yet these cells fail to maintain pmf under stress conditions. The two spatial subspecies therefore appear to be dynamically and functionally distinct with the polar clusters being associated with sensory function and the mobile complexes with maintenance of pmf.
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影响因子：
3.5
作者：
Cormack, BP;Valdivia, RH;Falkow, S
通讯作者：
Falkow, S
DOI：
10.1073/pnas.0402638101
发表时间：
2004-06-08
影响因子：
11.1
作者：
Gitai, Z;Dye, N;Shapiro, L
通讯作者：
Shapiro, L
影响因子：
11.4
作者：
Kleerebezem, M;Crielaard, W;Tommassen, J
通讯作者：
Tommassen, J
影响因子：
3.6
作者：
Kobayashi, Ryuji;Suzuki, Toshiharu;Yoshida, Masasuke
通讯作者：
Yoshida, Masasuke
影响因子：
10.5
作者：
Kruse, T;Blagoev, B;Gerdes, K
通讯作者：
Gerdes, K