Prokaryotic Diversity in the Rhizosphere of Organic, Intensive, and Transitional Coffee Farms in Brazil.
Prokaryotic Diversity in the Rhizosphere of Organic, Intensive, and Transitional Coffee Farms in Brazil.
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DOI：
10.1371/journal.pone.0106355
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ouverney CC
中科院分区：
文献类型：
--
作者：
Caldwell AC;Silva LC;da Silva CC;Ouverney CC
尽管过去 60 年来咖啡消费量不断增加，而且最近的研究表明咖啡种植对人类健康有积极的好处，但集约化咖啡种植方式却与环境破坏、人类健康风险和生物多样性减少有关。相比之下，有机农业已成为越来越受欢迎的替代方案，具有环境和健康效益。本研究旨在描述和确定三个巴西咖啡农场原核土壤微生物学的差异：一个实行集约化农业，一个实行有机农业，一个正在从集约化向有机农业转型。从三个农场各 20 个咖啡植物根际（直接受植物根系分泌物影响的土壤）和 10 个对照点（距离咖啡种植园 5 m 的土壤）采集土壤样品，总共 90 个样品。 16S rRNA 基因 V4 区域的分析揭示了所有三个农场中原核生物多样性的高水平，每个农场中都确定了数千个物种水平的可操作分类单位。此外，每个农场的咖啡根际微生物组以及咖啡根际土壤和对照土壤之间都存在统计学上的显着差异。发现与氮循环相关的两组原核生物，即古菌属 Candidatus Nitrososphaera 和细菌目根瘤菌目，在三个农场之间的组成在统计上存在差异，并且彼此呈反比关系。许多已知促进植物生长的固氮属数量很少（例如根瘤菌、农杆菌、醋杆菌、红螺菌、固氮螺菌），但它们所属的科在数据集中具有相对丰度最高的一些，这表明这些样本中可能存在许多新的菌群，可以作为潜在的植物生长促进细菌进一步研究，以提高咖啡产量，同时减少负面环境影响。
Despite a continuous rise in consumption of coffee over the past 60 years and recent studies showing positive benefits linked to human health, intensive coffee farming practices have been associated with environmental damage, risks to human health, and reductions in biodiversity. In contrast, organic farming has become an increasingly popular alternative, with both environmental and health benefits. This study aimed to characterize and determine the differences in the prokaryotic soil microbiology of three Brazilian coffee farms: one practicing intensive farming, one practicing organic farming, and one undergoing a transition from intensive to organic practices. Soil samples were collected from 20 coffee plant rhizospheres (soil directly influenced by the plant root exudates) and 10 control sites (soil 5 m away from the coffee plantation) at each of the three farms for a total of 90 samples. Profiling of 16S rRNA gene V4 regions revealed high levels of prokaryotic diversity in all three farms, with thousands of species level operational taxonomic units identified in each farm. Additionally, a statistically significant difference was found between each farm’s coffee rhizosphere microbiome, as well as between coffee rhizosphere soils and control soils. Two groups of prokaryotes associated with the nitrogen cycle, the archaeal genus Candidatus Nitrososphaera and the bacterial order Rhizobiales were found to be abundant and statistically different in composition between the three farms and in inverse relationship to each other. Many of the nitrogen-fixing genera known to enhance plant growth were found in low numbers (e.g. Rhizobium, Agrobacter, Acetobacter, Rhodospirillum, Azospirillum), but the families in which they belong had some of the highest relative abundance in the dataset, suggesting many new groups may exist in these samples that can be further studied as potential plant growth-promoting bacteria to improve coffee production while diminishing negative environmental impacts.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.2
作者：
Carvalho FM;Souza RC;Barcellos FG;Hungria M;Vasconcelos AT
通讯作者：
Vasconcelos AT
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
4
作者：
Carvalho, Dayene do Carmo;Pereira Lima Brigagao, Maisa Ribeiro;Azevedo, Luciana
通讯作者：
Azevedo, Luciana
影响因子：
--
作者：
Lima Filho, O. F. de;Malavolta, E.
通讯作者：
Malavolta, E.