Molecular Biologic Approach to the Diagnosis of Pancreatic Carcinoma Using Specimens Obtained by EUS-Guided Fine Needle Aspiration.
Molecular Biologic Approach to the Diagnosis of Pancreatic Carcinoma Using Specimens Obtained by EUS-Guided Fine Needle Aspiration.
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DOI：
10.1155/2012/243524
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发表时间：
2012
影响因子：
2
通讯作者：
Masaki T
中科院分区：
文献类型：
--
作者：
Kato K;Kamada H;Fujimori T;Aritomo Y;Ono M;Masaki T
我们回顾了内窥镜超声引导细针抽吸(EUS-FNA)的应用，EUS-FNA是一种快速、安全、经济、准确的胰腺肿瘤诊断方法。EUS-FNA目前用于胰腺肿瘤的诊断和分期。EUS-FNA诊断胰腺恶性肿瘤的敏感度为75%~94%，多数研究特异度接近100%。然而，EUS-FNA在诊断高分化或早期癌症方面有一定的局限性。最近的证据表明，使用EUS-FNA获得的标本进行分子生物学分析可以提高诊断的敏感性和特异性，特别是在细胞学交界性病例中。还有报道称，通过对EUS-FNA获得的样本进行基因分析，获得了关于患者对化疗的反应、手术可切除性、转移时间和总存活率的额外信息。其他研究表明，KRAS、MUC、P53、p16、S100P、Smad4和microRNAs的分析有助于胰腺癌的诊断。在这篇文章中，我们描述了用于胰腺疾病的EUS-FNA样本的遗传诊断技术的现状。我们还讨论了分子生物学分析在胰腺癌诊断中的作用。
We review the utility of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA), a rapid, safe, cost-effective, and accurate diagnostic modality for evaluating pancreatic tumors. EUS-FNA is currently used for the diagnosis and staging of pancreatic tumors. The sensitivity of EUS-FNA for pancreatic malignancy ranges from 75% to 94%, and its specificity approaches 100% in most studies. However, EUS-FNA has some limitations in the diagnosis of well-differentiated or early-stage cancers. Recent evidence suggests that molecular biological analysis using specimens obtained by EUS-FNA improves diagnostic sensitivity and specificity, especially in borderline cytological cases. It was also reported that additional information regarding patient response to chemotherapy, surgical resectability, time to metastasis, and overall survival was acquired from the genetic analysis of specimens obtained by EUS-FNA. Other studies have revealed that the analysis of KRAS, MUC, p53, p16, S100P, SMAD4, and microRNAs is helpful in making the diagnosis of pancreatic carcinoma. In this paper, we describe the present state of genetic diagnostic techniques for use with EUS-FNA samples in pancreatic diseases. We also discuss the role of molecular biological analyses for the diagnosis of pancreatic carcinoma.
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