Two novel, tightly linked, and rapidly evolving genes underlie Aedes aegypti mosquito reproductive resilience during drought.
Two novel, tightly linked, and rapidly evolving genes underlie Aedes aegypti mosquito reproductive resilience during drought.
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在干旱期间，两个小说，紧密联系和迅速发展的基因是埃及埃及蚊子生殖弹性的基础。
DOI：
10.7554/elife.80489
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发表时间：
2023-02-06
期刊：
影响因子：
7.7
通讯作者：
Vosshall LB
中科院分区：
文献类型：
--
作者：
Venkataraman K;Shai N;Lakhiani P;Zylka S;Zhao J;Herre M;Zeng J;Neal LA;Molina H;Zhao L;Vosshall LB
雌性埃及伊蚊作为多种病毒病原体的载体，给全球公共卫生带来了严重的负担。在最佳环境条件下，埃及伊蚊雌性可以接触到人类宿主，为卵子发育提供血液蛋白，同种雄性可以提供精子受精，淡水可以作为适合后代生存的产卵基质。随着全球气温上升，埃及伊蚊雌性面临着严重干旱和间歇性降水等气候挑战，这为产卵创造了不可预测的次优条件。在这里，我们表明，在实验室模拟的干旱条件下，雌性在卵巢中保留成熟的卵子很长一段时间，同时保持这些卵子的活力，直到它们可以在淡水中产卵。使用埃及伊蚊卵巢的转录组学和蛋白质组学分析，我们确定了两个以前未知的基因命名为tweedledee和tweedledum，每个编码一个小的，分泌的蛋白质，都显示卵巢富集，在卵滞留时间限制的表达。这些基因是蚊子特有的，在同线基因座内连接，并在正向选择下迅速进化，提高了它们具有适应功能的可能性。CRISPR-Cas9缺失tweedledee和tweedledum表明，它们是延长活卵保留期所特别需要的。这些结果突出了一个优雅的例子，分类限制基因的核心，一个重要的适应，装备埃及伊蚊女性与“保险”，灵活地延长其生殖时间表，而不失去生殖能力，从而使这个物种利用不可预测的栖息地在不断变化的世界。
Female Aedes aegypti mosquitoes impose a severe global public health burden as vectors of multiple viral pathogens. Under optimal environmental conditions, Aedes aegypti females have access to human hosts that provide blood proteins for egg development, conspecific males that provide sperm for fertilization, and freshwater that serves as an egg-laying substrate suitable for offspring survival. As global temperatures rise, Aedes aegypti females are faced with climate challenges like intense droughts and intermittent precipitation, which create unpredictable, suboptimal conditions for egg-laying. Here, we show that under drought-like conditions simulated in the laboratory, females retain mature eggs in their ovaries for extended periods, while maintaining the viability of these eggs until they can be laid in freshwater. Using transcriptomic and proteomic profiling of Aedes aegypti ovaries, we identify two previously uncharacterized genes named tweedledee and tweedledum, each encoding a small, secreted protein that both show ovary-enriched, temporally-restricted expression during egg retention. These genes are mosquito-specific, linked within a syntenic locus, and rapidly evolving under positive selection, raising the possibility that they serve an adaptive function. CRISPR-Cas9 deletion of both tweedledee and tweedledum demonstrates that they are specifically required for extended retention of viable eggs. These results highlight an elegant example of taxon-restricted genes at the heart of an important adaptation that equips Aedes aegypti females with ‘insurance’ to flexibly extend their reproductive schedule without losing reproductive capacity, thus allowing this species to exploit unpredictable habitats in a changing world.
影响因子：
4.4
作者：
Schmidt H;Greshake B;Feldmeyer B;Hankeln T;Pfenninger M
通讯作者：
Pfenninger M
埃及埃及蚊子的卵巢卵泡的渗透性。
DOI：
10.1083/jcb.50.1.201
发表时间：
1971-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Anderson WA;Spielman A
通讯作者：
Spielman A