Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model.
Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model.
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DOI：
10.1186/s12575-021-00159-3
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发表时间：
2021-11-11
影响因子：
6.4
通讯作者：
Asamura S
中科院分区：
文献类型：
--
作者：
Kumegawa S;Yamada G;Hashimoto D;Hirashima T;Kajimoto M;Isono K;Fujimoto K;Suzuki K;Uemura K;Ema M;Asamura S
由于在当今老龄化社会中慢性水肿病的发生率很高，对手术后水肿症的分析和详细观察变得越来越有必要。术后对包括淋巴管在内的动态血管系统进行检查以监测浮肿的形成并不是很有效。因此，研究这种血管系统的程序是必不可少的。通过将透明薄片植入小鼠尾部皮肤层作为一种新的手术模型(硅胶薄片介导的透明协议尾部水肿症；测试)，介绍并分析了一系列组织学分析，包括基于视频的LV观察和小鼠尾血管的三维组织重建。采用一种新的手术模型，通过一系列研究，观察了在水肿性恢复过程中，术后左心室主细(Neo)结缔组织结构的动态生成。从测试样品的现场双目图像记录中获得的快照图像表明存在主要的和拉长的细小(NEO)LV结构。结扎左心室后，证实左心室主干增大。在光片荧光显微镜(LSFM)观察的情况下，这种L.V.连接也是通过使用透明的3D样品来提出的。最后，在三维重建图像中提出了新生血管的生成，特别是在硅胶片邻近区域和手术边界区域。然而，直接检测细长(NEO)L.V.不适合通过这种LSFM和3D重建程序进行分析。因此，这种利用固定组织的方法适合于包括左心室在内的手术区域的一般观察。目前的手术方法和术后状态的分析是第一例用透明薄片观察体内血管的情况。包括FITC-葡聚糖介导的快照图像观察在内的系统分析表明，细小(Neo)淋巴管延长。术后分析包括LSFM和3D组织结构重建，适用于显示血管和淋巴管形成的固定结构。网上版载有补充材料，可在10.1186/s12575-021-00159-3查阅。
Because of the high frequency of chronic edema formation in the current “aged” society, analyses and detailed observation of post-surgical edema are getting more required. Post-surgical examination of the dynamic vasculature including L.V. (Lymphatic Vasculature) to monitor edema formation has not been efficiently performed. Hence, procedures for investigating such vasculature are essential. By inserting transparent sheet into the cutaneous layer of mouse tails as a novel surgery model (the Tail Edema by Silicone sheet mediated Transparency protocol; TEST), the novel procedures are introduced and analyzed by series of histological analyses including video-based L.V. observation and 3D histological reconstruction of vasculatures in mouse tails. The dynamic generation of post-surgical main and fine (neo) L.V. connective structure during the edematous recovery process was visualized by series of studies with a novel surgery model. Snapshot images taken from live binocular image recording for TEST samples suggested the presence of main and elongating fine (neo) L.V. structure. After the ligation of L.V., the enlargement of main L.V. was confirmed. In the case of light sheet fluorescence microscopy (LSFM) observation, such L.V. connections were also suggested by using transparent 3D samples. Finally, the generation of neo blood vessels particularly in the region adjacent to the silicone sheet and the operated boundary region was suggested in 3D reconstruction images. However, direct detection of elongating fine (neo) L.V. was not suitable for analysis by such LSFM and 3D reconstruction procedures. Thus, such methods utilizing fixed tissues are appropriate for general observation for the operated region including of L.V. The current surgical procedures and analysis on the post-surgical status are the first case to observe vasculatures in vivo with a transparent sheet. Systematic analyses including the FITC-dextran mediated snap shot images observation suggest the elongation of fine (neo) lymphatic vasculature. Post-surgical analyses including LSFM and 3D histological structural reconstruction, are suitable to reveal the fixed structures of blood and lymphatic vessels formation. The online version contains supplementary material available at 10.1186/s12575-021-00159-3.
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影响因子：
3.4
作者：
Hashimoto D;Kajimoto M;Ueda Y;Hyuga T;Fujimoto K;Inoue S;Suzuki K;Kataoka T;Kimura K;Yamada G
通讯作者：
Yamada G
影响因子：
1.5
作者：
Jorgensen, Mads Gustaf;Toyserkani, Navid Mohammadpour;Sorensen, Jens Ahm
通讯作者：
Sorensen, Jens Ahm
影响因子：
3.7
作者：
Hirashima T;Adachi T
通讯作者：
Adachi T
DOI：
10.1152/ajpheart.01019.2013
发表时间：
2014-05-01
影响因子：
4.8
作者：
Cuzzone, Daniel A.;Weitman, Evan S.;Mehrara, Babak J.
通讯作者：
Mehrara, Babak J.
影响因子：
2.8
作者：
Li, Zhongqiang;Hartzler, Thomas;Xu, Jian
通讯作者：
Xu, Jian