3D reconstruction and histopathological analyses on murine corporal body.
3D reconstruction and histopathological analyses on murine corporal body.
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DOI：
10.1002/rmb2.12369
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发表时间：
2021-04
影响因子：
3.4
通讯作者：
Yamada G
中科院分区：
文献类型：
--
作者：
Hashimoto D;Kajimoto M;Ueda Y;Hyuga T;Fujimoto K;Inoue S;Suzuki K;Kataoka T;Kimura K;Yamada G
勃起功能障碍（艾德）是随着老龄化社会的发展而日益增多的疾病之一。由于阴茎海绵体（CC）中窦状隙的复杂三维（3D）分布，对源自局部阴茎异常的艾德的基础了解甚少。因此，了解阴茎的3D组织结构是必要的。还对这种异常的调节信号的状态进行了分析。为了分析窦状隙的三维结构，对小鼠CC连续切片进行了三维重建。对年轻（2个月大）和老年（14个月大）CC进行组织学分析。对于老化CC的软骨形成信号传导状态，检查S 0X 9和RBPJK染色。窦状隙在邻近白蛋白图尼卡的CC外部区域显著发育。老化的CC样本在这些区域含有异位软骨细胞。软骨形成调节因子SOX 9的表达随着软骨细胞的出现而上调。Notch信号调节因子RBPJK在衰老CC中表达下调。窦状隙分布于CC的外侧，可能对勃起起重要作用。异位软骨形成的可能性引起的SOX 9/Notch信号随着年龄的变化。
Erectile dysfunction (ED) is one of the increasing diseases with aging society. The basis of ED derived from local penile abnormality is poorly understood because of the complex three‐dimensional (3D) distribution of sinusoids in corpus cavernosum (CC). Understanding the 3D histological structure of penis is thus necessary. Analyses on the status of regulatory signals for such abnormality are also performed. To analyze the 3D structure of sinusoid, 3D reconstruction from serial sections of murine CC were performed. Histological analyses between young (2 months old) and aged (14 months old) CC were performed. As for chondrogenic signaling status of aged CC, SOX9 and RBPJK staining was examined. Sinusoids prominently developed in the outer regions of CC adjacent to tunica albuginea. Aged CC samples contained ectopic chondrocytes in such regions. Associating with the appearance of chondrocytes, the expression of SOX9, chondrogenic regulator, was upregulated. The expression of RBPJK, one of the Notch signal regulators, was downregulated in the aged CC. Prominent sinusoids distribute in the outer region of CC which may possess important roles for erection. A possibility of ectopic chondrogenesis induced by alteration of SOX9/Notch signaling with aging is indicated.
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影响因子：
3.4
作者：
Hashimoto D;Kajimoto M;Ueda Y;Hyuga T;Fujimoto K;Inoue S;Suzuki K;Kataoka T;Kimura K;Yamada G
通讯作者：
Yamada G
影响因子：
10.5
作者：
Akiyama, H;Chaboissier, MC;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
影响因子：
3.5
作者：
Gonzalez-Cadavid, Nestor F.
通讯作者：
Gonzalez-Cadavid, Nestor F.
影响因子：
3.5
作者：
Ferrer, Julio Eduardo;Velez, Juan Diego;Herrera, Ana Milena
通讯作者：
Herrera, Ana Milena
影响因子：
4.6
作者：
Dong, Yufeng;Jesse, Alana M.;Hilton, Matthew J.
通讯作者：
Hilton, Matthew J.