Surface Plasmon Resonance Spectroscopy in Peptide and Protein Analysis
Surface Plasmon Resonance Spectroscopy in Peptide and Protein Analysis
复制标题
表面等离子体共振光谱在肽和蛋白质分析中的应用
DOI：
10.1002/9780470027318.a1633
复制
发表时间：
2006
影响因子：
6.1
通讯作者：
G. Tollin
中科院分区：
文献类型：
--
作者：
Z. Salamon;G. Tollin
本章详细概述了表面等离子体共振（SPR）光谱的理论基础，强调了原理和实际考虑，如SPR器件的薄膜设计，可以用于生物材料的表征，包括肽和蛋白质。结果表明，共振谱分析可以得到相互作用的生物分子各向异性膜的结构参数，包括质量分布、远程分子序和分子构象变化。还可以确定结合参数，包括结合亲和和速率常数。摘要综述了用于多肽和蛋白质分析的各种生物分子固定化技术。
This chapter presents a detailed overview of the theoretical basis of surface plasmon resonance (SPR) spectroscopy, emphasizing both principles and practical considerations such as thin-film designs for SPR devices which can be employed in characterization of biological materials, including peptides and proteins. It is demonstrated that the analysis of the resonance spectrum yields structural parameters of anisotropic films of interacting biomolecules, including distribution of mass, long-range molecular order, and molecular conformational changes. Binding parameters, including binding affinities and rate constants, can also be determined. A summary is presented of different techniques for biomolecule immobilization on SPR-generating surfaces applicable in peptide and protein analysis.
通过表面等离子体共振光谱探测视紫红质的构象变化。
影响因子：
2.9
作者：
Salamon,Z;Wang,Y;Brown,MF;Macleod,HA;Tollin,G
通讯作者：
Tollin,G
DOI：
10.1073/pnas.81.19.6159
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BRIAN, AA;MCCONNELL, HM
通讯作者：
MCCONNELL, HM