Animal Models of Urologic Chronic Pelvic Pain Syndromes: Findings From the Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network.
Animal Models of Urologic Chronic Pelvic Pain Syndromes: Findings From the Multidisciplinary Approach to the Study of Chronic Pelvic Pain Research Network.
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DOI：
10.1016/j.urology.2015.03.007
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发表时间：
2015-06
期刊：
影响因子：
2.1
通讯作者：
Klumpp DJ
中科院分区：
文献类型：
--
作者：
Lai H;Gereau RW 4th;Luo Y;O'Donnell M;Rudick CN;Pontari M;Mullins C;Klumpp DJ
描述MAPP（慢性盆腔疼痛研究的多学科方法）研究网络研究人员为提高UCPPS（泌尿系慢性盆腔疼痛综合征）动物模型的实用性所采取的方法。代表基础科学和临床专业知识的多学科研究小组定义了UCPPS啮齿动物模型的关键表型标准。UCPPS症状根据其临床意义进行优先排序。开发了量化动物与患者症状相关性的方法。在提出的啮齿动物模型中实施所述方法，以评价和比较动物与人类生殖学相关的表型特征。骨盆疼痛和尿频被认为是人类UCPPS的主要特征，并优先用于动物评估。使用内脏对膀胱扩张的反应，并通过将von Frey细丝应用于下腹部（称为触觉异常性疼痛）来量化伤害性感受。使用排尿笼或印迹垫测定法评估排尿活性为自由排尿，并通过膀胱测压法评估对膀胱充盈的反应。模型在表征深度和再现盆腔疼痛的程度以及UCPPS的尿频特征方面各不相同。反映人类UCPPS的多个关键特征的啮齿动物模型可以被识别，并为机制研究提供增强的临床意义。我们已经开发了一种策略，以评估当前和未来的动物模型的UCPPS的基础上，人类生殖学。这种方法为改善动物机制研究和临床研究之间的转换提供了基础，并作为一种验证策略，用于评估模型的有效性，为未知病因的疾病驱动的疾病。
To describe the approach taken by MAPP (Multi-Disciplinary Approach to the Study of Chronic Pelvic Pain) Research Network investigators to advance the utility of UCPPS (urologic chronic pelvic pain syndromes) animal models. A multidisciplinary team of investigators representing basic science and clinical expertise defined key phenotypic criteria for rodent models of UCPPS. UCPPS symptoms were prioritized based on their clinical significance. Methods for quantifying animal correlates to patient symptoms were developed. The methods were implemented across proposed rodent models for evaluation and comparison of animals for phenotypic characteristics relevant to human symptomatology. Pelvic pain and urinary frequency were deemed primary features of human UCPPS and were prioritized for assessment in animals. Nociception was quantified using visceromotor response to bladder distention, and by applying von Frey filaments to the lower abdomen (referred tactile allodynia). Micturition activity was assessed as free voiding using micturition cages or blotting pad assays, and in response to bladder filling by cystometry. Models varied in both depth of characterization and degree of recapitulating pelvic pain and urinary frequency characteristics of UCPPS. Rodent models that reflect multiple, key characteristics of human UCPPS may be identified and provide enhanced clinical significance to mechanistic studies. We have developed a strategy for evaluating current and future animal models of UCPPS based on human symptomatology. This approach provides a foundation for improved translation between mechanistic studies in animals and clinical research, and serves as a validation strategy for assessing validity of models for symptom-driven disorders of unknown etiology.
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影响因子：
2.9
作者：
Robbins, M. T.;DeBerry, J.;Ness, T. J.
通讯作者：
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