Ultrastructural cytoskeleton alterations and modification of actin expression in the NIH/3T3 cell line after transformation with Ha-ras-activated oncogene.
Ultrastructural cytoskeleton alterations and modification of actin expression in the NIH/3T3 cell line after transformation with Ha-ras-activated oncogene.
复制标题
Ha-ras 激活癌基因转化后 NIH/3T3 细胞系中超微结构细胞骨架的改变和肌动蛋白表达的修饰。
DOI：
--
复制
发表时间：
1990
影响因子：
--
通讯作者：
S. Modina
中科院分区：
文献类型：
--
作者：
L. Lombardi;D. Ballinari;I. Bongarzone;M. Migliari;P. Mondellini;C. Traversari;S. Modina
用免疫荧光、免疫电子显微镜和肌动蛋白亚型免疫电泳法研究了Ha-ras激活癌基因对NIH/3T3成纤维细胞单层细胞骨架的影响。转化灶由圆形的细胞和稀有的应力纤维组成，稀疏散布，形成少见的灶性接触和纤维连接。罗丹明-鬼臼糖苷染色和荧光标记的抗非肌肉细胞肌动蛋白抗体证实转化细胞中的应力纤维丢失。免疫电子显微镜显示，转化的细胞通过含有纤维连接蛋白的细丝显著地固定在底物上。免疫荧光和免疫印迹分析观察到α-肌动蛋白异构体的下调。α-肌动蛋白的弥漫性分布缺乏与应激纤维的特定关联，挑战了α-肌动蛋白下调与应力纤维丢失之间存在联系的假说。
Cytoskeleton alterations of NIH/3T3 fibroblast monolayers transfected with Ha-ras-activated oncogene were studied by immunofluorescence, immunoelectron microscopy, and immunoelectrophoretic analysis of actin isoforms. Transformation foci were found to consist of cells with a round shape and rare stress fibers that spread sparsely, forming rare focal contacts and fibronexuses. The loss of stress fibers in transformed cells was confirmed by staining with rhodamine-phalloidin and with a fluorescinated anti-non-muscle cell actin antibody. The transformed cells were anchored to the substrate prominently by filaments that contained fibronectin, as showed by immunoelectron microscopy. A down-regulation of alpha-actin isoform was observed by immunofluorescence and immunoblotting analysis using a specific monoclonal antibody. The diffuse distribution of alpha-actin, lacking a specific association with stress fibers, challenges the hypothesis of a connection between alpha-actin down-regulation and stress fiber loss.
影响因子：
56.9
作者：
BARSAGI, D;FERAMISCO, JR
通讯作者：
FERAMISCO, JR