(1)H MRS detection of glycine residue of reduced glutathione in vivo.
(1)H MRS detection of glycine residue of reduced glutathione in vivo.
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DOI：
10.1016/j.jmr.2009.11.013
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发表时间：
2010-02
期刊：
影响因子：
--
通讯作者：
Young K
中科院分区：
文献类型：
--
作者：
Kaiser LG;Marjańska M;Matson GB;Iltis I;Bush SD;Soher BJ;Mueller S;Young K
谷胱甘肽 (GSH) 是一种强大的抗氧化剂，存在于不同种类的细胞中，包括中枢神经系统的细胞。使用 1H MR 光谱法检测人脑中的 GSH 会受到低浓度以及与其他代谢物光谱重叠的阻碍。以前的 MRS 方法主要侧重于通过编辑方案检测半胱氨酸残基 (GSH-Cys)。本研究重点检测生理 pH 和温度下与谷氨酸和谷氨酰胺 (Glx) 重叠的甘氨酸残基 (GSH-Gly)。该研究的首要目标是获得用于表征生理条件下 GSH-Gly 信号的光谱参数。第二个目标是研究一种在体内将 GSH-Gly 与 Glx 分离的新方法。通过利用数值模拟以及大范围磁场（4.0-14 T）的实验来表征信号。所提出的分离方案利用 J 差异编辑来量化 Glx 贡献，以将其与 GSH-Gly 信号分离。所提出的方法保留了 100% 的 GSH-Gly 信号。与之前使用的针对 GSH-Cys 残基的方法相比，计算出目标共振的信噪比总体增加，从而产生显着的 SNR 改善。这使得人体临床研究的采集时间更短。
Glutathione (GSH) is a powerful antioxidant found inside different kinds of cells, including those of the central nervous system. Detection of GSH in the human brain using 1H MR spectroscopy is hindered by low concentration and spectral overlap with other metabolites. Previous MRS methods focused mainly on the detection of the cysteine residue (GSH-Cys) via editing schemes. This study focuses on the detection of the glycine residue (GSH-Gly), which is overlapped by glutamate and glutamine (Glx) under physiological pH and temperature. The first goal of the study was to obtain the spectral parameters for characterization of the GSH-Gly signal under physiological conditions. The second goal was to investigate a new method of separating GSH-Gly from Glx in vivo. The characterization of the signal was carried out by utilization of numerical simulations as well as experiments over a wide range of magnetic fields (4.0–14 T). The proposed separation scheme utilizes J-difference editing to quantify the Glx contribution to separate it from the GSH-Gly signal. The presented method retains 100% of the GSH-Gly signal. The overall increase in signal to noise ratio of the targeted resonance is calculated to yield a significant SNR improvement compared to previously used methods that target GSH-Cys residue. This allows shorter acquisition times for in vivo human clinical studies.
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影响因子：
2.2
作者：
Garwood, M;DelaBarre, L
通讯作者：
DelaBarre, L
影响因子：
4
作者：
FUJIWARA, S;FORMICKAKOZLOWSKA, G;KOZLOWSKI, H
通讯作者：
KOZLOWSKI, H
影响因子：
3.3
作者：
Terpstra, Melissa;Marjanska, Malgorzata;Gruetter, Rolf
通讯作者：
Gruetter, Rolf
影响因子：
3.3
作者：
Kaiser, Lana G.;Young, Karl;Matson, Gerald B.
通讯作者：
Matson, Gerald B.
影响因子：
2.9
作者：
Kaiser, L. G.;Young, K.;Matson, G. B.
通讯作者：
Matson, G. B.