Transcriptome and proteome research in veterinary science: what is possible and what questions can be asked?
Transcriptome and proteome research in veterinary science: what is possible and what questions can be asked?
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DOI：
10.1100/2012/254962
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发表时间：
2012
影响因子：
--
通讯作者：
Gruber AD
中科院分区：
文献类型：
--
作者：
Klopfleisch R;Gruber AD
近年来，用于转录组和蛋白质组的完整分析的几种技术已经达到允许其作为科学工具常规应用的技术水平。这些方法的原理是鉴定和定量组织中多达数万种RNA和蛋白质种类，与常规方法如PCR和Western印迹的顺序分析相反。由于技术进步，转录组和蛋白质组分析在生物研究的各个领域变得越来越重要。它们主要用于探索性鉴定疾病相关的复杂基因表达模式，从而为假设驱动的研究奠定基础。本文综述了目前转录组分析的主要方法，即微阵列、Ref-Seq、定量PCR阵列，并讨论了它们的潜力和局限性。其次，介绍了目前蛋白质组研究的主要方法，即二维凝胶电泳法、鸟枪法蛋白质组学、MudPIT法以及各种技术概念。最后，实验策略的生物标志物的发现，实验环境中的预后基因集和探索与假设驱动的方法，用于阐明疾病相关的基因和分子途径的识别进行了描述，并强调其在兽医研究中的研究潜力。
In recent years several technologies for the complete analysis of the transcriptome and proteome have reached a technological level which allows their routine application as scientific tools. The principle of these methods is the identification and quantification of up to ten thousands of RNA and proteins species in a tissue, in contrast to the sequential analysis of conventional methods such as PCR and Western blotting. Due to their technical progress transcriptome and proteome analyses are becoming increasingly relevant in all fields of biological research. They are mainly used for the explorative identification of disease associated complex gene expression patterns and thereby set the stage for hypothesis-driven studies. This review gives an overview on the methods currently available for transcriptome analysis, that is, microarrays, Ref-Seq, quantitative PCR arrays and discusses their potentials and limitations. Second, the most powerful current approaches to proteome analysis are introduced, that is, 2D-gel electrophoresis, shotgun proteomics, MudPIT and the diverse technological concepts are reviewed. Finally, experimental strategies for biomarker discovery, experimental settings for the identification of prognostic gene sets and explorative versus hypothesis driven approaches for the elucidation of diseases associated genes and molecular pathways are described and their potential for studies in veterinary research is highlighted.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
14.9
作者：
Griffiths-Jones, S
通讯作者：
Griffiths-Jones, S
影响因子：
48
作者：
Cloonan, Nicole;Forrest, Alistair R. R.;Grimmond, Sean M.
通讯作者：
Grimmond, Sean M.
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.
影响因子：
14.9
作者：
Griffiths-Jones S;Grocock RJ;van Dongen S;Bateman A;Enright AJ
通讯作者：
Enright AJ