Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes.
Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes.
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DOI：
10.1186/1471-2350-14-93
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发表时间：
2013-09-20
影响因子：
--
通讯作者：
Henriques JA
中科院分区：
文献类型：
--
作者：
da Silva AL;da Rosa HT;Karnopp TE;Charlier CF;Ellwanger JH;Moura DJ;Possuelo LG;Valim AR;Guecheva TN;Henriques JA
We investigated a potential link between genetic polymorphisms in genes XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr) with the level of DNA damage and repair, accessed by comet and micronucleus test, in 51 COPD patients and 51 controls. Peripheral blood was used to perform the alkaline and neutral comet assay; and genetic polymorphisms by PCR/RFLP. To assess the susceptibility to exogenous DNA damage, the cells were treated with methyl methanesulphonate for 1-h or 3-h. After 3-h treatment the % residual damage was calculated assuming the value of 1-h treatment as 100%. The cytogenetic damage was evaluated by buccal micronucleus cytome assay (BMCyt). COPD patients with the risk allele XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) showed higher DNA damage by comet assay. The residual damage was higher for COPD with risk allele in the four genes. In COPD patients was showed negative correlation between BMCyt (binucleated, nuclear bud, condensed chromatin and karyorrhexic cells) with pulmonary function and some variant genotypes. Our results suggest a possible association between variant genotypes in XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr), DNA damage and progression of COPD.
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DOI：
10.4161/cc.7.18.6679
发表时间：
2008-09-15
期刊：
Cell cycle (Georgetown, Tex.)
影响因子：
--
作者：
Mao Z;Bozzella M;Seluanov A;Gorbunova V
通讯作者：
Gorbunova V
DOI：
10.1016/j.ijrobp.2004.12.027
发表时间：
2005-07-15
影响因子：
7
作者：
De Ruyck, K;Van Eijkeren, M;Thierens, H
通讯作者：
Thierens, H
影响因子：
4.3
作者：
Duarte, Marcia Cristina;Colombo, Jucimara;Silva, Ana Elizabete
通讯作者：
Silva, Ana Elizabete
DOI：
10.1016/s1383-5742(03)00013-9
发表时间：
2003-03-01
影响因子：
5.3
作者：
Bonassi, S;Neri, M;Fenech, M
通讯作者：
Fenech, M
影响因子：
2.7
作者：
Hoffmann, H;Isner, C;Speit, G
通讯作者：
Speit, G