Involvement of Regulatory Interactions among Global Regulators GlxR, SugR, and RamA in Expression of ramA in Corynebacterium glutamicum
Involvement of Regulatory Interactions among Global Regulators GlxR, SugR, and RamA in Expression of ramA in Corynebacterium glutamicum
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全局调节因子 GlxR、SugR 和 RamA 之间的调节相互作用参与谷氨酸棒杆菌中 ramA 的表达
DOI：
10.1128/jb.00016-13
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发表时间：
2013
影响因子：
3.2
通讯作者：
H. Yukawa
中科院分区：
文献类型：
--
作者：
Koichi Toyoda;H. Teramoto;W. Gunji;M. Inui;H. Yukawa
谷氨酸棒杆菌的碳代谢中心基因受一个由多个全局调控因子组成的转录调控网络控制。已知编码这些调节子之一的拉马的启动子区在体外和/或体内与其基因产物拉马以及与另外两种调节子GlxR和SugR相互作用。虽然拉马已被证实抑制其自身的表达，但GlxR和SugR在拉马表达中的作用仍不清楚。在这项研究中，我们研究了GlxR结合位点失活对拉马启动子-lacZ融合体表达的影响，在sugR和拉马的单和双缺失突变体的遗传背景下。在野生型背景下，拉马启动子活性降低到无法检测的水平，通过引入突变到GlxR结合位点，但增加了sugR缺失，表明GlxR和SugR的功能作为转录激活因子和阻遏物，分别。GlxR结合位点突变对拉马启动子活性的显著抑制在很大程度上通过sugR和/或拉马的缺失来补偿。此外，ramA-sugR双突变体中的拉马启动子活性与拉马突变体中的启动子活性相当，但显著高于sugR突变体中的启动子活性。综上所述，拉马表达水平可能通过GlxR依赖性激活和拉马沿着SugR的阻遏动态平衡，以响应细胞外和/或细胞内条件的扰动。这些发现为C.谷氨酸。
ABSTRACT The central carbon metabolism genes in Corynebacterium glutamicum are under the control of a transcriptional regulatory network composed of several global regulators. It is known that the promoter region of ramA, encoding one of these regulators, interacts with its gene product, RamA, as well as with the two other regulators, GlxR and SugR, in vitro and/or in vivo. Although RamA has been confirmed to repress its own expression, the roles of GlxR and SugR in ramA expression have remained unclear. In this study, we examined the effects of GlxR binding site inactivation on expression of the ramA promoter-lacZ fusion in the genetic background of single and double deletion mutants of sugR and ramA. In the wild-type background, the ramA promoter activity was reduced to undetectable levels by the introduction of mutations into the GlxR binding site but increased by sugR deletion, indicating that GlxR and SugR function as the transcriptional activator and repressor, respectively. The marked repression of ramA promoter activity by the GlxR binding site mutations was largely compensated for by deletions of sugR and/or ramA. Furthermore, ramA promoter activity in the ramA-sugR double mutant was comparable to that in the ramA mutant but was significantly higher than that in the sugR mutant. Taken together, it is likely that the level of ramA expression is dynamically balanced by GlxR-dependent activation and repression by RamA along with SugR in response to perturbation of extracellular and/or intracellular conditions. These findings add multiple regulatory loops to the transcriptional regulatory network model in C. glutamicum.
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“1972政权”日本安保体系下的台湾（中文）
DOI：
--
发表时间：
2022
期刊：
影响因子：
--
作者：
Barrett;B;Ohta;H.;and Trencher;G. "Prospects for Acceleration of Socio-Technical Transitions for Deep Decarbonization," pp. 400-414;青山瑠妙;福田円;舒旻「中国：EUとの脆弱な相互依存」pp. 247-271;福田円;青山瑠妙;青山瑠妙;青山瑠妙;福田円;青山瑠妙;福田円
通讯作者：
福田円
影响因子：
1.5
作者：
H. Yukawa;Crispinus A. Omumasaba;H. Nonaka;P. Kós;N. Okai;N. Suzuki;Masako Suda;Y. Tsuge;J. Watanabe;Yoko Ikeda;A. Vertès;M. Inui
通讯作者：
H. Yukawa;Crispinus A. Omumasaba;H. Nonaka;P. Kós;N. Okai;N. Suzuki;Masako Suda;Y. Tsuge;J. Watanabe;Yoko Ikeda;A. Vertès;M. Inui
影响因子：
1.6
作者：
Hayashi, M;Mizoguchi, H;Ikeda, M
通讯作者：
Ikeda, M
影响因子：
30.8
作者：
Shen-Orr, SS;Milo, R;Alon, U
通讯作者：
Alon, U