MiR-543 Inhibits the Migration and Epithelial-To-Mesenchymal Transition of TGF-β-Treated Endometrial Stromal Cells via the MAPK and Wnt/β-Catenin Signaling Pathways.
MiR-543 Inhibits the Migration and Epithelial-To-Mesenchymal Transition of TGF-β-Treated Endometrial Stromal Cells via the MAPK and Wnt/β-Catenin Signaling Pathways.
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MiR-543 通过 MAPK 和 Wnt/β-连环蛋白信号通路抑制 TGF-β 处理的子宫内膜基质细胞的迁移和上皮间质转化
DOI：
10.3389/pore.2021.1609761
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Wang L;Liu D;Wei J;Yuan L;Zhao S;Huang Y;Ma J;Yang Z
Intrauterine adhesion (IUA) is one of the most prevalent reproductive system diseases in females. MicroRNAs (miRNAs) are reported to be master regulators in a variety of diseases, including IUA, but the role of microRNA-543 (miR-543) in IUA remains to be elucidated. In this study, we observed that miR-543 was downregulated in transforming growth factor-beta (TGF-β)-treated endometrial stromal cells (ESCs). Functionally, we observed that miR-543 suppressed the migration, epithelial-to-mesenchymal transition (EMT), and inhibited expression of extracellular matrix (ECM) proteins in TGF-β-treated ESCs. Mechanistically, MAPK1 is targeted by miR-543 after prediction and screening. A luciferase reporter assay demonstrated that miR-543 complementarily binds with the 3′ untranslated region of mitogen-activated protein kinase 1 (MAPK1), and western blot analysis indicated that miR-543 negatively regulates MAPK1 protein levels. In addition, results from rescue assays showed that miR-543 inhibits the migration and EMT of TGF-β-treated ESCs by targeting MAPK1. In addition, we observed that miR-543 inactivates the Wnt/β-catenin signaling pathway through inhibiting the phosphorylation of MAPK1 and β-catenin. Finally, we confirmed that miR-543 represses migration, EMT and inhibits levels of ECM proteins in TGF-β-treated ESCs by targeting the Wnt/β-catenin signaling pathway. Our results demonstrated that miR-543 suppresses migration and EMT of TGF-β-treated ESCs by targeting the MAPK and Wnt/β-catenin pathways.
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影响因子：
11
作者：
Cao J;Huang M;Guo L;Zhu L;Hou J;Zhang L;Pero A;Ng S;El Gaamouch F;Elder G;Sano M;Goate A;Tcw J;Haroutunian V;Zhang B;Cai D
通讯作者：
Cai D
影响因子：
4.8
作者：
Krikun, G;Mor, G;Lockwood, CJ
通讯作者：
Lockwood, CJ
DOI：
10.1091/mbc.1.12.875
发表时间：
1990-11-01
期刊：
CELL REGULATION
影响因子：
--
作者：
SPORN, MB;ROBERTS, AB
通讯作者：
ROBERTS, AB
影响因子：
--
作者：
Du, Yang;Zhu, Heng-cheng;Xiao, Cheng-cheng
通讯作者：
Xiao, Cheng-cheng
宫腔粘连中 HMGA2 诱导的上皮-间质转化被 let-7d 逆转
影响因子：
4
作者：
Song M;Cao C;Zhou Z;Yao S;Jiang P;Wang H;Zhao G;Hu Y
通讯作者：
Hu Y