Overexpression of AhpC enhances stress tolerance and N2-fixation in Anabaena by upregulating stress responsive genes.
Overexpression of AhpC enhances stress tolerance and N2-fixation in Anabaena by upregulating stress responsive genes.
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AhpC 的过表达通过上调应激反应基因增强鱼腥藻的应激耐受性和 N2 固定
DOI：
10.1016/j.bbagen.2016.07.031
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Rai LC
中科院分区：
文献类型：
--
作者：
Shrivastava AK;Pandey S;Dietz KJ;Singh PK;Singh S;Rai LC
探讨了过氧化物对鱼腥藻7120固定CO2和N2能力的调节作用。为此，产生了鱼腥藻菌株，其携带额外拷贝的ahpC（An + ahpC）或通过从其内源性功能ahpC（AnΔahpC）中缺失而产生。An + ahpC中的AhpC水平比野生型高2.2至6.0倍。An + ahpC对光系统I和II、固氮酶、超氧化物歧化酶和过氧化氢酶活性的表达上调了1.4- 2倍，而插入突变体（AnΔahpC）的活性下调了1.3- 2.5倍。An + ahpC中过氧化物、超氧阴离子和丙二醛含量较低，而AnΔahpC中含量较高。在选定的胁迫下，AnΔahpC中的生长被抑制约40-60%，而An + ahpC中的生长被增强33-40%。最有趣的是，在An + ahpC中异形胞频率增加。为了解决转录和转录后效应，包括groEL，fld，kat，戈尔，gst，dps，bfr，tf，sodA，dnaK，prx，uspA，pcs和apx的基因的转录物被定量，发现在未应激和应激An + ahpC中分别增加了1.33- 7.70倍和1.76- 13.80倍。以匡威的方式，它们在未应激的AnΔahpC中下调1.20- 7.50倍，在应激的AnΔahpC中下调1.23 - 10.20倍。可以得出结论，AhpC的水平在正常和应激条件下控制一组主要的代谢和发育基因，因此可能是鱼腥藻氧化还原调节系统的核心。
The study explores the significance of peroxides in regulating the CO2- and N2-fixation capacities in Anabaena sp. PCC7120. To this end Anabaena strains were generated carrying an extra copy ofahpC(An + ahpC) or by deleting from their endogenous functionalahpC(AnΔahpC). AhpC levels were 2.2- to 6.0-fold higher in An + ahpC than in wild type. An + ahpC revealed 1.4- to 2-fold upregulation of photosystems I and II, nitrogenase, superoxide dismutase and catalase activities while same activities were 1.3- to 2.5-fold downregulated in the insertional mutant (AnΔahpC) compared to the wild type. Peroxide, superoxide and malondialdehyde contents were low in An + ahpC and high in AnΔahpC. Growth was inhibited in AnΔahpC by approximately 40–60% compared to a 33–40% enhanced growth in An + ahpC under selected stresses. Most interestingly, heterocyst frequency was increased in An + ahpC. In order to address transcriptional and posttranscriptional effects, transcripts of genes includinggroEL,fld,kat,gor,gst, dps, bfr, tf, sodA, dnaK,prx, uspA,pcs and apx were quantified and found to be increased 1.33- to 7.70-fold in unstressed and 1.76- to 13.80-fold in stressed An + ahpC. In a converse manner, they were downregulated by 1.20- to 7.50-fold in unstressed and 1.23 to 10.20-fold in stressed AnΔahpC. It is concluded that the level of AhpC controls a major set of metabolic and developmental genes in normal and stress conditions and thus likely is in the core of the redox regulatory system ofAnabaena.
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