Increased SPC24 in prostatic diseases and diagnostic value of SPC24 and its interacting partners in prostate cancer.
Increased SPC24 in prostatic diseases and diagnostic value of SPC24 and its interacting partners in prostate cancer.
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SPC24 在前列腺疾病中的增加以及 SPC24 及其相互作用伙伴在前列腺癌中的诊断价值
DOI：
10.3892/etm.2021.10355
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发表时间：
2021-09
影响因子：
2.7
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Chen S;Wang X;Zheng S;Li H;Qin S;Liu J;Jia W;Shao M;Tan Y;Liang H;Song W;Lu S;Liu C;Yang X
SPC 24是肿瘤发生中有丝分裂检查点机制的重要组成部分。在各种癌症中发现了高水平的SPC 24，包括乳腺癌、肺癌、肝癌、骨肉瘤和甲状腺癌。然而，据我们所知，SPC 24对前列腺癌（PCa）和其他前列腺疾病的影响仍不清楚。在本研究中，在癌症基因组图谱（TCGA）数据库中包含的PCa患者亚组中评估了整体SPC 24信使RNA（mRNA）的表达。SPC 24在>60岁的PCa患者中的表达水平高于<60岁的患者，且SPC 24的表达水平与前列腺特异性抗原（PSA）和淋巴结转移有关（P <0.05）。SPC 24高表达与PCa阴性预后相关（P<0.05）。此外，在中国前列腺炎，良性前列腺肥大（BPH）和PCa患者中，SPC 24的表达水平显着高于相邻/正常组织，通过逆转录-定量聚合酶链反应，免疫组化和蛋白质印迹法进行评估。SPC 24的高表达与高Gleason分期相关（IV和V; P<0.05）。基于基因本体论和途径功能富集分析的进一步分析表明，核分裂周期80（NDC 80），SPC 24蛋白相互作用伴侣，和有丝分裂纺锤体检查点丝氨酸/苏氨酸蛋白激酶BUB 1（BUB 1），纺锤体组装检查点的核心亚基，可能与PCa发育中的SPC 24相关。最后，使用二元逻辑回归，结合SPC 24和BUB 1或NDC 80之间的受试者操作特征的算法表明，这些标志物的组合可以提供比其他PCa诊断标志物更好的PCa诊断能力。总之，这些发现表明SPC 24可能是一种有前途的前列腺疾病生物标志物。
SPC24 is a crucial component of the mitotic checkpoint machinery in tumorigenesis. High levels of SPC24 have been found in various cancers, including breast cancer, lung cancer, liver cancer, osteosarcoma and thyroid cancer. However, to the best of our knowledge, the impact of SPC24 on prostate cancer (PCa) and other prostate diseases remains unclear. In the present study expression of global SPC24 messenger RNA (mRNA) was assessed in a subset of patients with PCa included in The Cancer Genome Atlas (TCGA) database. Increased levels of SPC24 expression were found in PCa patients >60 years old compared to patients <60 and increased SPC24 expression was also associated with higher levels of prostate specific antigen (P<0.05) and lymph node metastasis (P<0.05). Higher levels of SPC24 expression were associated with negative outcomes in PCa patients (P<0.05). Furthermore, in Chinese patients with prostatitis, benign prostatic hypertrophy (BPH) and PCa, SPC24 was expressed at significantly higher levels than that in adjacent/normal tissues, as assessed by reverse transcription-quantitative polymerase chain reaction, immunohistochemistry and western blotting. High expression of SPC24 was associated with high Gleason stages (IV and V; P<0.05). Further analysis, based on Gene Ontology and pathway functional enrichment analysis, suggested that nuclear division cycle 80 (NDC80), an SPC24 protein interaction partner, and mitotic spindle checkpoint serine/threonine-protein kinase BUB1 (BUB1), a core subunit of the spindle assembly checkpoint, may be associated with SPC24 in PCa development. Finally, using binary logistic regression, algorithms combining the receiver operating characteristic between SPC24 and BUB1 or NDC80 indicated that a combination of these markers may provide better PCa diagnosis ability than other PCa diagnosis markers. Taken together, these findings suggest that SPC24 may be a promising prostate disease biomarker.
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DOI：
10.1083/jcb.201012035
发表时间：
2011-06-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ricke RM;Jeganathan KB;van Deursen JM
通讯作者：
van Deursen JM
影响因子：
8.8
作者：
Hung SC;Lai SW;Tsai PY;Chen PC;Wu HC;Lin WH;Sung FC
通讯作者：
Sung FC
DOI：
10.1186/s13046-015-0127-8
发表时间：
2015-02-06
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Merola R;Tomao L;Antenucci A;Sperduti I;Sentinelli S;Masi S;Mandoj C;Orlandi G;Papalia R;Guaglianone S;Costantini M;Cusumano G;Cigliana G;Ascenzi P;Gallucci M;Conti L
通讯作者：
Conti L
影响因子：
2.1
作者：
Marks, Leonard S.;Fradet, Yves;Groskopf, Jack
通讯作者：
Groskopf, Jack
影响因子：
1.6
作者：
Mao Z;Ji A;Yang K;He W;Hu Y;Zhang Q;Zhang D;Xie L
通讯作者：
Xie L