Multiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensors.
Multiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensors.
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DOI：
10.1007/s00424-012-1175-y
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发表时间：
2013-03
影响因子：
4.5
通讯作者：
Zhang, Jin
中科院分区：
文献类型：
--
作者：
Depry, Charlene;Mehta, Sohum;Zhang, Jin
细胞依赖于一个复杂的、相互连接的信号通路网络来感知和解释细胞外环境的变化。基于基因编码的荧光蛋白(FP)生物传感器的发展使研究人员能够直接观察和表征活细胞中这些细胞内信号通路的时空动力学。然而，当单独监测单个信号事件时，关于交叉通路之间精确的时间和空间关系的详细信息往往会丢失。随着生物传感器技术的不断发展，同时成像多个基于FP的生物传感器变得越来越可行，通过实现对同一细胞内不同信号事件的并行监控，可以更深入地了解细胞内信号网络的复杂协调。在这篇综述中，我们讨论了基于FP的生物传感器的多路成像的几种策略，同时也强调了与这些技术相关的一些挑战，并强调了可能导致细胞内信号事件并行监测的进一步改进的其他途径。
Cells rely on a complex, interconnected network of signaling pathways to sense and interpret changes in their extracellular environment. The development of genetically encoded fluorescent protein (FP)-based biosensors has made it possible for researchers to directly observe and characterize the spatiotemporal dynamics of these intracellular signaling pathways in living cells. However, detailed information regarding the precise temporal and spatial relationships between intersecting pathways is often lost when individual signaling events are monitored in isolation. As the development of biosensor technology continues to advance, it is becoming increasingly feasible to image multiple FP-based biosensors concurrently, permitting greater insights into the intricate coordination of intracellular signaling networks by enabling parallel monitoring of distinct signaling events within the same cell. In this review, we discuss several strategies for multiplexed imaging of FP-based biosensors, while also underscoring some of the challenges associated with these techniques and highlighting additional avenues that could lead to further improvements in parallel monitoring of intracellular signaling events.
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