A demonstration of pattern formation without positional information in Dictyostelium
A demonstration of pattern formation without positional information in Dictyostelium
复制标题
盘基网柄菌在没有位置信息的情况下图案形成的演示
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
R. Kay
中科院分区：
文献类型：
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作者：
C. Thompson;S. Reichelt;R. Kay
尽管由形态发生素梯度传递的位置信息是在发育过程中形成模式的一种广泛接受的方式，但可以想象另一种方法，该方法基于具有不同命运的细胞的混合分化，然后将它们分类为离散的模式元素。有人提出盘基网柄菌前柄和前孢子细胞在发育的丘阶段以这种方式表现。然而，很难最终证明它们最初是混合分化的，因为土丘内细胞的快速运动使得不可能确定前柄和前孢子细胞的起源。我们采取了一种新方法来解决这个问题，即使用肌动蛋白解聚药物 latrunculin-A 来阻断发育不同阶段的细胞运动。在这种瘫痪的结构中，前茎和前孢子细胞以基本正常的效率和时间分化。当运动被足够早地阻止时，主要细胞类型随后都会在整个聚集体中的分散位置分化，甚至在通向聚集体的流中也是如此。我们的工作强烈支持这样的观点，即盘基网柄菌的前柄/前孢子模式在没有位置信息的情况下形成，并证明 latrunculin-A 可能为研究其他生物体的模式提供有用的工具。
Although positional information, conveyed by morphogen gradients, is a widely accepted way of forming patterns during development, an alternative method is conceivable, based on the intermingled differentiation of cells with different fates, followed by their sorting into discrete pattern elements. It has been proposed that Dictyostelium prestalk and prespore cells behave in this way at the mound stage of development. However, it has been difficult to conclusively demonstrate that they initially differentiate intermingled, because rapid cell movement within the mound makes it impossible to be sure where prestalk and prespore cells originate. We have taken a novel approach to address this problem by blocking cell movement at different stages in development, using the actin‐depolymerizing drug, latrunculin‐A. Prestalk and prespore cells differentiate with essentially normal efficiency and timing in such paralyzed structures. When movement is blocked sufficiently early, the major cell types all subsequently differentiate at scattered positions throughout the aggregate, and even in the streams leading into it. Our work strongly supports the idea that the prestalk/prespore pattern in Dictyostelium forms without positional information and demonstrate that latrunculin‐A may provide a useful tool for the investigation of patterning in other organisms.
盘基网柄菌中的丘细胞运动和形态发生。
DOI：
10.1006/dbio.1999.9208
发表时间：
1999
期刊：
Developmental biology.
影响因子：
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作者：
Kellerman,KA;McNally,JG
通讯作者：
McNally,JG