MiR393 regulation of auxin signaling and redox-related components during acclimation to salinity in Arabidopsis.
MiR393 regulation of auxin signaling and redox-related components during acclimation to salinity in Arabidopsis.
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DOI：
10.1371/journal.pone.0107678
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Casalongué CA
中科院分区：
文献类型：
--
作者：
Iglesias MJ;Terrile MC;Windels D;Lombardo MC;Bartoli CG;Vazquez F;Estelle M;Casalongué CA
植物可塑性的一个最引人注目的方面是响应环境变化的发育调节。植物的生长和发育在很大程度上依赖于植物激素生长素，生长素通过部分冗余的F-box受体家族（TIR 1-AFB）发挥其功能。我们以前曾报道，拟南芥双突变体tir 1 afb 2是更耐盐胁迫比野生型植物，我们推测，生长素信号的下调可能是拟南芥适应盐度。在这项工作中，我们表明，NaCl介导的盐胁迫诱导miR 393的表达，通过增强AtMIR 393 A的转录，并导致同时减少TIR 1和AFB 2受体的水平。因此，NaCl触发Aux/IAA阻遏物的稳定，导致生长素信号的下调。此外，我们报告说，miR 393可能参与抑制侧根（LR）的启动，出现和伸长在盐度，因为mir 393 ab突变体显示出减少抑制的紧急和成熟LR的数量和长度NaCl处理后。此外，mir 393 ab突变体植物具有增加的活性氧（ROS）在LR的水平，并降低抗坏血酸过氧化物酶（APX）的酶活性与野生型植物相比，在盐度。因此，miR 393对TIR 1和AFB 2受体的调控可能是拟南芥在盐胁迫下协调组织和时间特异性生长反应和耐受性的生长素信号和特异性氧化还原相关组分之间的关键检查点。
One of the most striking aspects of plant plasticity is the modulation of development in response to environmental changes. Plant growth and development largely depend on the phytohormone auxin that exerts its function through a partially redundant family of F-box receptors, the TIR1-AFBs. We have previously reported that the Arabidopsis double mutant tir1 afb2 is more tolerant to salt stress than wild-type plants and we hypothesized that down-regulation of auxin signaling might be part of Arabidopsis acclimation to salinity. In this work, we show that NaCl-mediated salt stress induces miR393 expression by enhancing the transcription of AtMIR393A and leads to a concomitant reduction in the levels of the TIR1 and AFB2 receptors. Consequently, NaCl triggers stabilization of Aux/IAA repressors leading to down-regulation of auxin signaling. Further, we report that miR393 is likely involved in repression of lateral root (LR) initiation, emergence and elongation during salinity, since the mir393ab mutant shows reduced inhibition of emergent and mature LR number and length upon NaCl-treatment. Additionally, mir393ab mutant plants have increased levels of reactive oxygen species (ROS) in LRs, and reduced ascorbate peroxidase (APX) enzymatic activity compared with wild-type plants during salinity. Thus, miR393 regulation of the TIR1 and AFB2 receptors could be a critical checkpoint between auxin signaling and specfic redox-associated components in order to coordinate tissue and time-specific growth responses and tolerance during acclimation to salinity in Arabidopsis.
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