LINC-PINT Suppresses the Aggressiveness of Thyroid Cancer by Downregulating miR-767-5p to Induce TET2 Expression.
LINC-PINT Suppresses the Aggressiveness of Thyroid Cancer by Downregulating miR-767-5p to Induce TET2 Expression.
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LINC-PINT 通过下调 miR-767-5p 诱导 TET2 表达来抑制甲状腺癌的侵袭性
DOI：
10.1016/j.omtn.2020.05.033
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发表时间：
2020-12-04
期刊：
影响因子：
--
通讯作者：
Lu X
中科院分区：
文献类型：
--
作者：
Jia M;Li Z;Pan M;Tao M;Wang J;Lu X
长链非编码RNA（lncRNA）长基因间非蛋白编码RNA，p53诱导转录物（LINC-PINT）在肺癌和结肠癌细胞中显示出抗侵袭活性。然而，LINC-PINT在甲状腺癌中的作用尚不清楚。在本工作中，我们探讨了LINC-PINT在60对甲状腺癌和癌旁正常组织中的表达。探讨LINC-PINT在甲状腺癌中的临床意义和生物学功能。LINC-PINT表达在甲状腺癌中相对于邻近正常组织下调（p = 0.0002）。LINC-PINT的低表达与晚期肿瘤淋巴结转移（TNM）分期（p = 0.0306）和淋巴结转移（p = 0.0359）显著相关。LINC-PINT的异位表达抑制甲状腺癌细胞的增殖、侵袭和肿瘤发生。在机制上，LINC-PINT与microRNA（miR）-767-5p相关并下调。此外，LINC-PINT过表达减轻了miR-767- 5 p介导的TET 2抑制。miR-767- 5 p促进甲状腺癌的侵袭性，这可被TET 2的过表达逆转。miR-767- 5 p的共表达或TET 2的消耗挽救了LINC-PINT对甲状腺癌细胞增殖和侵袭的抑制作用。此外，甲状腺癌中miR-767- 5 p和LINC-PINT之间存在负相关性（r =-0.34772，p = 0.01789）。总之，LINC-PINT通过miR-767- 5 p/TET 2轴作为甲状腺癌的肿瘤抑制因子发挥作用，代表了甲状腺癌的潜在治疗靶点。长链非编码RNA LINC-PINT在甲状腺癌中起肿瘤抑制作用。其下调有助于甲状腺癌的生长和进展。LINC-PINT的恢复为甲状腺癌提供了一种潜在的治疗策略。
Long noncoding RNA (lncRNA) long intergenic nonprotein-coding RNA, p53-induced transcript (LINC-PINT) has shown anti-invasive activity in lung and colon cancer cells. However, the role of LINC-PINT in thyroid cancer is unclear. In the present work, we explored the expression of LINC-PINT in 60 paired thyroid cancer and adjacent normal tissues. The clinical significance and biological function of LINC-PINT in thyroid cancer were determined. LINC-PINT expression was downregulated in thyroid cancer relative to adjacent normal tissues (p = 0.0002). Low expression of LINC-PINT was significantly associated with advanced tumor node metastasis (TNM) stage (p = 0.0306) and lymph node metastasis (p = 0.0359). Ectopic expression of LINC-PINT suppressed the proliferation, invasion, and tumorigenesis of thyroid cancer cells. Mechanistically, LINC-PINT associated with and downregulated microRNA (miR)-767-5p. Moreover, LINC-PINT overexpression relieved miR-767-5p-mediated repression of ten-eleven translocation 2 (TET2). miR-767-5p promoted aggressiveness of thyroid cancer, which was reversed by overexpression of TET2. Coexpression of miR-767-5p or depletion of TET2 rescued the inhibitory effect of LINC-PINT on thyroid cancer cell proliferation and invasion. In addition, there was a negative correlation between miR-767-5p and LINC-PINT in thyroid cancer (r = −0.34772, p = 0.01789). Taken together, LINC-PINT functions as a tumor suppressor in thyroid cancer via the miR-767-5p/TET2 axis, representing a potential therapeutic target for thyroid cancer. Long noncoding RNA LINC-PINT functions as a tumor suppressor in thyroid cancer. Its downregulation contributes to thyroid cancer growth and progression. Restoration of LINC-PINT provides a potential therapeutic strategy against thyroid cancer.
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影响因子：
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作者：
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