Alkaline Phosphatase of Human Periodontal Ligament Fibroblast-Like Cells
Alkaline Phosphatase of Human Periodontal Ligament Fibroblast-Like Cells
复制标题
人牙周膜成纤维样细胞的碱性磷酸酶
作者：
T. Kawase;S. Sato;K. Miake;S. Saito
建立、维持人牙周韧带成纤维细胞（HPLF）和人牙槽骨细胞（HABC）的培养，并对骨细胞的标记酶碱性磷酸酶（ALPase）活性进行表征。在增殖期，HPLF和HABC的ALPase未检测到。但在融合阶段，HPLF合成的ALPase水平与HABC相似。此外，5 × 10-9 mol/L 1,25 (OH)2-维生素D3 （1,25 (OH)2-D3）显著刺激HPLF的ALPase活性（7.5倍）。因此，由于HPLF的ALPase活性与成骨细胞相似，人牙周韧带成纤维细胞可能具有成骨细胞的功能。
Cultures of human periodontal ligament fibroblasts (HPLF) and human alveolar bone cells (HABC) were established, maintained, and characterized with respect to alkaline phosphatase (ALPase) activity, a marker enzyme of bone cells. During the proliferative phase, ALPase of HPLF and HABC were not detectable. But at the confluent stage, HPLF synthesized levels of ALPase similar to HABC. Also, ALPase activity of HPLF was markedly stimulated (7.5-fold) by 5 x 10-9 mol/L 1,25 (OH)2-vitamin D3 (1,25 (OH)2-D3). Therefore, since ALPase activity of HPLF resembles that present in osteoblasts, human periodontal ligament fibroblasts may function as "osteoblastic" fibroblasts.
DOI：
10.1016/s0021-9258(18)34781-1
发表时间：
1982-04
期刊：
The Journal of biological chemistry
影响因子：
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作者：
R. Majeska;G. Rodan
通讯作者：
R. Majeska;G. Rodan