Proton NMR studies of .lambda. cro repressor. 2. Sequential resonance assignments of the proton NMR spectrum
Proton NMR studies of .lambda. cro repressor. 2. Sequential resonance assignments of the proton NMR spectrum
复制标题
λ 的质子核磁共振研究。
DOI：
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
B. Reid
中科院分区：
文献类型：
--
作者：
P. L. Weber;D. Wemmer;B. Reid
用二维核磁共振波谱研究了噬菌体Lambda的CRO抑制蛋白在溶液中的结构。参考文献[Wothrich，K.，Wide，G.，Wagner，G.，&Braun，W.(1982)J.Mol.比奥尔。155,311-319]，通过J相关光谱(COSY)确定了各个自旋系统，必要时还补充了中继相干转移光谱(RELAY)。核奥弗豪瑟效应谱(NOESY)被用来获得序列特异性分配。从二维谱中指定了66个氨基酸中65个的主链共振(NH和CαH)，以及大部分的侧链共振。所指定的质子在35℃、10 mM磷酸二氢钾、pH 6.8、10 mM磷酸二氢钾、pH 4.6、0.2M KCl和0.1 mM EDTA中发生了化学位移。在加入盐后，观察到了一些共振的微小位移，但没有看到光谱中的重大变化，表明这些离子强度之间没有发生整体结构变化。在初级序列的不同区域中可以看到具有α-螺旋、β-链和转角特征的NOE图案。从这些区域的位置来看，CrO在溶液中的二级结构似乎与晶体结构几乎相同[Anderson，W.F.，Ohlendorf，D.H.，Takeda，Y.，&Matthews，B.W.(1981)Nature(London)290,754-758]。遗漏的指认包括Pro-59共振和8个赖氨酸、3个精氨酸和5个异亮氨酸残基中的3个的外围质子。
: The cro repressor protein from bacteriophage lambda has been studied in solution by two-dimensional nuclear magnetic resonance spectroscopy (2D NMR). Following the approach of Wuthrich and co-workers [Wuthrich, K., Wider, G., Wagner, G., & Braun, W. (1982) J. Mol. Biol. 155, 311-319], individual spin systems were identified by J-correlated spectroscopy (COSY) supplemented, where necessary, by relayed coherence transfer spectroscopy (RELAY). Nuclear Overhauser effect spectroscopy (NOESY) was used to obtain sequence-specific assignments. From the two-dimensional spectra, the peptide backbone resonances (NH and C alpha H) for 65 of the 66 amino acids were assigned, as well as most of the side chain resonances. The chemical shifts for the assigned protons are reported at 35 degrees C in 10 mM potassium phosphate, pH 6.8, and in 10 mM potassium phosphate, pH 4.6, 0.2 M KCl, and 0.1 mM EDTA. Small shifts were observed for some resonances upon addition of salt, but no major changes in the spectrum were seen, indicating that no global structural change occurs between these ionic strengths. NOE patterns characteristic of alpha-helices, beta-strands, and turns are seen in various regions of the primary sequence. From the location of these regions the secondary structure of cro in solution appears to be virtually identical with the crystal structure [Anderson, W. F., Ohlendorf, D. H., Takeda, Y., & Matthews, B. W. (1981) Nature (London) 290, 754-758]. Missing assignments include the Pro-59 resonances and the peripheral protons of the eight lysine, the three arginine, and three of the five isoleucine residues.
影响因子：
5.6
作者：
Wemmer,DE;Chou,SH;Reid,BR
通讯作者：
Reid,BR
lambda 噬菌体 OR3 脱氧核糖核酸片段的质子核磁共振谱中的亚氨基质子分配。
影响因子：
2.9
作者：
Ulrich,EL;John,EM;Gough,GR;Brunden,MJ;Gilham,PT;Westler,WM;Markley,JL
通讯作者：
Markley,JL