Metabolomic assessment of embryo viability.
Metabolomic assessment of embryo viability.
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DOI：
10.1055/s-0033-1363556
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发表时间：
2014-03
影响因子：
2.7
通讯作者：
Seli E
中科院分区：
文献类型：
--
作者：
Uyar A;Seli E
着床前胚胎代谢表现出与胚胎发育潜力相关的独特特征。在此基础上，我们假设培养基的代谢物含量反映了单个胚胎的着床潜力。这一假设在连续的研究中得到了验证，报道了培养基代谢物与胚胎发育或临床妊娠之间的显著关联。对无创、可靠和快速的胚胎评估策略的需求促进了体外受精（IVF）代谢组学研究，以提高单胚胎移植的成功率。随着分析技术和生物信息学的发展，利用剩余培养基的光谱分析来预测胚胎活力的商用仪器已经开发出来。然而，尽管最初的主要研究结果很有希望，但最近使用商业仪器进行的随机对照试验未能显示出代谢组学作为形态学辅助手段在提高妊娠率方面的一致益处。目前，代谢组学技术在临床IVF实验室的应用，需要在可能的情况下消除导致结果不一致的因素，并建立可靠的预测模型，考虑胚胎选择过程中所有可能的偏差来源。
Preimplantation embryo metabolism demonstrates distinctive characteristics associated with the developmental potential of embryos. On this basis, metabolite content of culture media was hypothesized to reflect the implantation potential of individual embryos. This hypothesis was tested in consecutive studies reporting a significant association between culture media metabolites and embryo development or clinical pregnancy. The need for a noninvasive, reliable, and rapid embryo assessment strategy promoted metabolomics studies in vitro fertilization (IVF) in an effort to increase success rates of single embryo transfers. With the advance of analytical techniques and bioinformatics, commercial instruments were developed to predict embryo viability using spectroscopic analysis of surplus culture media. However, despite the initial promising results from proof-of-principal studies, recent randomized controlled trials using commercial instruments failed to show a consistent benefit in improving pregnancy rates when metabolomics is used as an adjunct to morphology. At present, the application of metabolomics technology in clinical IVF laboratory requires the elimination of factors underlying inconsistent findings, when possible, and development of reliable predictive models accounting for all possible sources of bias throughout the embryo selection process.
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影响因子：
6.1
作者：
Hardarson, T.;Ahlstrom, A.;Wikland, M.
通讯作者：
Wikland, M.
影响因子：
4
作者：
Botros L;Sakkas D;Seli E
通讯作者：
Seli E
影响因子：
13.1
作者：
Dunn, WB;Ellis, DI
通讯作者：
Ellis, DI
影响因子：
64.8
作者：
CLEGG, KB;PIKO, L
通讯作者：
PIKO, L
DOI：
10.1530/jrf.0.0930245
发表时间：
1991-09-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
BRISON, DR;LEESE, HJ
通讯作者：
LEESE, HJ