Global expression profiling reveals genetic programs underlying the developmental divergence between mouse and human embryogenesis.
Global expression profiling reveals genetic programs underlying the developmental divergence between mouse and human embryogenesis.
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全局表达谱揭示了小鼠和人类胚胎发生之间发育差异的遗传程序
DOI：
10.1186/1471-2164-14-568
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发表时间：
2013-08-20
期刊：
影响因子：
4.4
通讯作者：
Li WX
中科院分区：
文献类型：
--
作者：
Xue L;Cai JY;Ma J;Huang Z;Guo MX;Fu LZ;Shi YB;Li WX
背景小鼠与人类的相似性使其成为研究人类发育和疾病的理想模型。在过去的几十年里，小鼠转基因和基因敲除研究的进展极大地加强了这种模型的使用，并显着提高了我们对发育过程中基因功能的理解。最近，全球基因表达分析揭示了早期胚胎发育到原肠胚形成阶段的新特征，并确实提供了支持人类和小鼠早期发育保守的分子证据。另一方面，很少的信息是已知的基因调控网络，管理随后的器官发生。重要的是，小鼠和人类的发育在器官发生过程中存在差异。例如，小鼠胚胎出生在器官发生的末期，而在人类中，大多数器官的持续生长和成熟的后续胎儿期跨越了人类胚胎发生的2/3以上。虽然最近的两项研究报告的基因表达谱在人类器官发生，没有全球基因表达分析已经做了小鼠organogenesis.ResultsHere，我们报告了一个详细的分析全球基因表达谱从鸡蛋到结束的小鼠器官发生。我们的研究揭示了不同的时间调控模式属于不同的功能（基因本体论或GO）类别，支持他们在器官发生过程中的作用的基因。更重要的是，比较分析确定了小鼠和人类器官发生中的保守和差异基因调控程序，后者可能是两个物种之间发育差异的原因，并进一步提出了脊椎动物进化过程中一种新的发育策略。结论我们在这里报道了第一个基因组-整个小鼠胚胎发生的广泛基因表达分析，并比较小鼠和人类胚胎发生期间的转录组图谱。鉴于我们早期的观察，在给定的过程中，基因的功能往往是发育共同调节在器官形成过程中，我们的微阵列数据在这里应该有助于识别与小鼠发育相关的基因和/或推断未知基因的发育功能。此外，我们的研究可能有助于探讨脊椎动物进化的分子基础。
BackgroundMouse has served as an excellent model for studying human development and diseases due to its similarity to human. Advances in transgenic and knockout studies in mouse have dramatically strengthened the use of this model and significantly improved our understanding of gene function during development in the past few decades. More recently, global gene expression analyses have revealed novel features in early embryogenesis up to gastrulation stages and have indeed provided molecular evidence supporting the conservation in early development in human and mouse. On the other hand, little information is known about the gene regulatory networks governing the subsequent organogenesis. Importantly, mouse and human development diverges during organogenesis. For instance, the mouse embryo is born around the end of organogenesis while in human the subsequent fetal period of ongoing growth and maturation of most organs spans more than 2/3 of human embryogenesis. While two recent studies reported the gene expression profiles during human organogenesis, no global gene expression analysis had been done for mouse organogenesis.ResultsHere we report a detailed analysis of the global gene expression profiles from egg to the end of organogenesis in mouse. Our studies have revealed distinct temporal regulation patterns for genes belonging to different functional (Gene Ontology or GO) categories that support their roles during organogenesis. More importantly, comparative analyses identify both conserved and divergent gene regulation programs in mouse and human organogenesis, with the latter likely responsible for the developmental divergence between the two species, and further suggest a novel developmental strategy during vertebrate evolution.ConclusionsWe have reported here the first genome-wide gene expression analysis of the entire mouse embryogenesis and compared the transcriptome atlas during mouse and human embryogenesis. Given our earlier observation that genes function in a given process tends to be developmentally co-regulated during organogenesis, our microarray data here should help to identify genes associated with mouse development and/or infer the developmental functions of unknown genes. In addition, our study might be useful for invesgtigating the molecular basis of vertebrate evolution.
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影响因子：
14.9
作者：
Blake JA;Bult CJ;Kadin JA;Richardson JE;Eppig JT;Mouse Genome Database Group
通讯作者：
Mouse Genome Database Group
影响因子：
14.9
作者：
Nueda MJ;Carbonell J;Medina I;Dopazo J;Conesa A
通讯作者：
Conesa A
影响因子：
9.8
作者：
Church DM;Goodstadt L;Hillier LW;Zody MC;Goldstein S;She X;Bult CJ;Agarwala R;Cherry JL;DiCuccio M;Hlavina W;Kapustin Y;Meric P;Maglott D;Birtle Z;Marques AC;Graves T;Zhou S;Teague B;Potamousis K;Churas C;Place M;Herschleb J;Runnheim R;Forrest D;Amos-Landgraf J;Schwartz DC;Cheng Z;Lindblad-Toh K;Eichler EE;Ponting CP;Mouse Genome Sequencing Consortium
通讯作者：
Mouse Genome Sequencing Consortium
DOI：
10.1073/pnas.0402597101
发表时间：
2004-07-13
影响因子：
11.1
作者：
Hamatani, T;Daikoku, T;Dey, SK
通讯作者：
Dey, SK
DOI：
10.1073/pnas.0603227103
发表时间：
2006-09-19
影响因子：
11.1
作者：
Kocabas, Arif Murat;Crosby, Javier;Cibelli, Jose Bernardo
通讯作者：
Cibelli, Jose Bernardo