Expression of tyrosinase genes associated with fruiting body formation and pigmentation in Grifola frondosa
Expression of tyrosinase genes associated with fruiting body formation and pigmentation in Grifola frondosa
复制标题
灰树花子实体形成和色素沉着相关酪氨酸酶基因的表达
DOI：
10.1016/j.myc.2019.04.003
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发表时间：
2019
期刊：
影响因子：
1.4
通讯作者：
Aimi Tadanori
中科院分区：
文献类型：
--
作者：
Kawaguchi Nobuhisa;Hayashi Mirai;Nakano Shota;Shimomura Norihiro;Yamaguchi Takeshi;Aimi Tadanori
从灰树花子实体中分离得到一种棕色素，其理化性质（包括紫外光谱）与真菌黑色素基本一致。从G. frondosa中，鉴定了参与这种多孔蘑菇的黑色素生物合成途径的基因，包括酪氨酸酶（tyr 1和tyr 2）。转录分析表明，只有在原始阶段的tyr2的表达显着的变化。使用抗酪氨酸酶抗体的免疫电镜显示TYR2定位于原基的细胞壁中。因此，TYR2可能与该多孔菌G. frondosa，黑色素可能产生于细胞壁。
Chemical and physical properties (including ultraviolet spectrum) of a brown pigment isolated from Grifola frondosa fruiting bodies were almost identical to those of fungal melanin. From the full genome sequence of G. frondosa, genes involved in the melanin biosynthesis pathway of this polypore mushroom were identified, including tyrosinases (tyr1 and tyr2). Transcriptional analyses showed dramatic changes only in the expression of tyr2 in the primordial stage. Immuno-electron microscopy using anti-tyrosinase antibodies showed that TYR2 localizes in the cell walls of primordia. Therefore, TYR2 may be closely associated with melanin biosynthesis in this polypore mushroom G. frondosa, and melanin might be produced in the cell wall.
黑色素
DOI：
--
发表时间：
2021
期刊：
Encyclopedia of Astrobiology
影响因子：
--
作者：
Marco d'Ischia;P. Manini
通讯作者：
P. Manini
影响因子：
1.4
作者：
S. Kanda;T. Aimi;S. Masumoto;K. Nakano;Y. Kitamoto;T. Morinaga
通讯作者：
T. Morinaga
影响因子：
5.6
作者：
ALTSCHUL, SF;GISH, W;LIPMAN, DJ
通讯作者：
LIPMAN, DJ