Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye.
Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye.
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DOI：
10.1371/journal.pone.0093450
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Yamaguchi M
中科院分区：
文献类型：
--
作者：
Vo N;Taga A;Inaba Y;Yoshida H;Cotterill S;Yamaguchi M
微小染色体维持蛋白10（Mcm 10）是一种重要的蛋白质，它是一种在S.酿酒酵母对果蝇和人类的作用，并且是DNA复制起始所必需的。果蝇Mcm 10（dMcm 10）基因敲低的RNA干扰在眼睛的成虫盘诱导异常的眼睛形态（粗糙的眼睛表型），并在成年人的眼睛小眼数量减少。我们还观察到dMcm 10敲低果蝇品系的眼盘中的S相和M相的延迟。这些结果表明dMcm 10在S期和M期的进展中起重要作用。此外，基因组损伤和细胞凋亡诱导dMcm 10敲低在眼睛的光盘。令人惊讶的是，当我们使用deadpan-lacZ和klingon-lacZ增强子捕获线来监测眼盘中的感光细胞时，通过GMR-GAL 4驱动器敲低dMcm 10减少了R7感光细胞的信号。这些数据表明dMcm 10参与R7细胞分化。这种参与似乎是独立的dMcm 10敲低诱导的细胞凋亡。总之，这些结果表明dMcm 10敲低对DNA复制和R7细胞分化有影响。
Mini chromosome maintenance 10 (Mcm10) is an essential protein, which is conserved from S. cerevisiae to Drosophila and human, and is required for the initiation of DNA replication. Knockdown of Drosophila Mcm10 (dMcm10) by RNA interference in eye imaginal discs induces abnormal eye morphology (rough eye phenotype), and the number of ommatidia is decreased in adult eyes. We also observed a delay in the S phase and M phase in eye discs of dMcm10 knockdown fly lines. These results show important roles for dMcm10 in the progression of S and M phases. Furthermore, genome damage and apoptosis were induced by dMcm10 knockdown in eye imaginal discs. Surprisingly, when we used deadpan-lacZ and klingon-lacZ enhancer trap lines to monitor the photoreceptor cells in eye discs, knockdown of dMcm10 by the GMR-GAL4 driver reduced the signals of R7 photoreceptor cells. These data suggest an involvement of dMcm10 in R7 cell differentiation. This involvement appears to be independent of the apoptosis induced by dMcm10 knockdown. Together, these results suggest that dMcm10 knockdown has an effect on DNA replication and R7 cell differentiation.
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DOI：
10.1016/j.bbaexp.2006.07.002
发表时间：
2006-07-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Hayashi, Yuko;Kato, Masaki;Yamaguchi, Masamitsu
通讯作者：
Yamaguchi, Masamitsu
影响因子：
16
作者：
Wohlschlegel, JA;Dhar, SK;Walter, JC
通讯作者：
Walter, JC
影响因子：
4.8
作者：
Izumi, M;Yatagai, F;Hanaoka, F
通讯作者：
Hanaoka, F
影响因子：
3.3
作者：
Liachko, I;Tye, BK
通讯作者：
Tye, BK
影响因子：
1.5
作者：
Reichhart, JM;Ligoxygakis, P;Gubb, D
通讯作者：
Gubb, D