Low vasa vasorum densities correlate with inflammation and subintimal thickening: potential role in location--determination of atherogenesis.
Low vasa vasorum densities correlate with inflammation and subintimal thickening: potential role in location--determination of atherogenesis.
复制标题
DOI：
10.1016/j.atherosclerosis.2009.03.010
复制
发表时间：
2009-10
期刊：
影响因子：
5.3
通讯作者：
Ritman, E. L.
中科院分区：
文献类型：
--
作者：
Goessl, M.;Versari, D.;Lerman, L. O.;Chade, A. R.;Beighley, P. E.;Erbel, R.;Ritman, E. L.
目的：评价冠状动脉血管(VV)的空间分布在确定早期动脉粥样硬化病变发展位置中的作用。6头3月龄雌性杂交猪在安乐死前饲喂2%高胆固醇(HC)饲料3个月。另外6头猪在整个6个月期间都饲喂正常日粮(N)。取右冠状动脉，用MicroCT(20μm立方体素大小)扫描。扫描后，随机选取组织切片进行核因子-κ-B、缺氧诱导因子-1α(α-1)、巨噬细胞、血管性血友病因子、二氢乙锭、肿瘤坏死因子-α(肿瘤坏死因子-α)和白介素6(IL-6)染色。将冠状动脉壁四个等象限的阳性染色细胞的数量以及内膜/中层比率与从Micro-CT图像(与组织切片的位置非常匹配)获得的VV密度(#/mm2)进行比较。在正常和正常猪中，NF-κB(r=0.73和0.70)、HIF-1α(r=0.74和0.77)、α(r=0.58和0.72)和IL-6(r=0.70和0.72)阳性细胞数以及DHE(肯德尔-陶系数−0.64和−0.63)的表达与VV密度呈负相关。HC组VV密度与内膜/中膜比值呈负相关(r=0.65)。我们的数据表明，冠状动脉壁内的低VV密度区域容易受到缺氧、氧化应激和微炎症的影响，因此可能是早期动脉粥样硬化形成的起点。
To assess the role of coronary vasa vasorum (VV) spatial distribution in determining the location of early atherosclerotic lesion development. Six, 3-month old, female, crossbred swine were fed 2% high-cholesterol (HC) diet for 3 months prior to euthanasia. Six other pigs were fed normal diet (N) for the entire 6 months. Right coronary arteries were harvested and scanned intact with micro-CT (20μm cubic-voxel-size). After scanning, randomly selected cross-sectional histological sections were stained for nuclear-factor kappaB (NF-κB), hypoxia-inducible factor-1alpha (HIF-1α), macrophages, von-Willebrand-factor, dihydroethidium (DHE), tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6). The number of positive stained cells, as well as intima-to-media ratio, were compared with VV density (#/mm2) obtained from micro-CT images (which closely matched the location of the histological sections) in each of four equal quadrants of the coronary vessel wall. In normal, as well as HC pigs, the number of NF-κB (r=0.73 and 0.70), HIF-1α (r=0.74 and 0.77), TNF-α (r=0.58 and 0.72) and IL-6 (r=0.70 and 0.72) positive cells as well as the expression of DHE (Kendall tau coefficient −0.64 and −0.63) inversely correlated with VV density. In HC the VV density also inversely correlated with intima/media ratios (r=0.65). Our data suggest that low-VV-density-territories within the coronary vessel wall are susceptible to hypoxia, oxidative stress and microinflammation and may therefore be starting points of early atherogenesis.
登录
查看更多内容
DOI：
10.1152/ajpheart.00399.2003
发表时间：
2003-11-01
影响因子：
4.8
作者：
Gössl, M;Malyar, NM;Ritman, EL
通讯作者：
Ritman, EL
影响因子：
37.8
作者：
RAKUSAN, K;FLANAGAN, MF;VANPRAAGH, R
通讯作者：
VANPRAAGH, R
影响因子：
37.8
作者：
Chade, AR;Rodriguez-Porcel, M;Lerman, LO
通讯作者：
Lerman, LO
影响因子：
10.8
作者：
Rodriguez-Porcel, M;Lerman, LO;Lerman, A
通讯作者：
Lerman, A
影响因子：
37.8
作者：
Moulton, KS;Heller, E;Folkman, J
通讯作者：
Folkman, J