Investigating endogenous peptides and peptidases using peptidomics.
Investigating endogenous peptides and peptidases using peptidomics.
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DOI：
10.1021/bi200417k
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发表时间：
2011-09-06
期刊：
影响因子：
2.9
通讯作者：
Saghatelian, Alan
中科院分区：
文献类型：
--
作者：
Tinoco, Arthur D.;Saghatelian, Alan
肽已被证明是重要的生物活性分子，而不仅仅是蛋白质降解产物。生物活性肽在体内充当激素、神经递质和抗菌剂。生物活性肽信号传导的失调也被认为与疾病有关，靶向肽激素途径已经成为开发新疗法的成功策略。生物活性肽在生物学中的重要性促使人们研究这些分子的功能和调控。肽分析的经典方法依赖于靶向免疫测定，但某些科学问题需要对肽组（细胞、组织或生物体中的所有肽）进行更广泛和更详细的观察。在这篇综述中，我们讨论了肽组学如何通过应用先进的液相色谱-串联质谱（LC-MS/MS）方法来满足这一需求，这些方法为肽的活性和调节提供了独特的见解。
Rather than simply being protein degradation products, peptides have proven to be important bioactive molecules. Bioactive peptides act as hormones, neurotransmitters and antimicrobial agents in vivo. The dysregulation of bioactive peptide signaling is also known to be involved in disease, and targeting peptide hormone pathways has been successful strategy in the development of novel therapeutics. The importance of bioactive peptides in biology has spurred research to elucidate the function and regulation of these molecules. Classical methods for peptide analysis have relied on targeted immunoassays, but certain scientific questions necessitated a broader and more detailed view of the peptidome–all the peptides in a cell, tissue or organism. In this review we discuss how peptidomics has emerged to fill this need through the application of advanced liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods that provide unique insights into peptide activity and regulation.
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