The plasma exosomal miR-1180-3p serves as a novel potential diagnostic marker for cutaneous melanoma.
The plasma exosomal miR-1180-3p serves as a novel potential diagnostic marker for cutaneous melanoma.
复制标题
血浆外泌体 miR-1180-3p 可作为皮肤黑色素瘤的新型潜在诊断标志物
DOI：
10.1186/s12935-021-02164-8
复制
发表时间：
2021-09-20
影响因子：
5.8
通讯作者：
Peng C
中科院分区：
文献类型：
--
作者：
Guo Y;Zhang X;Wang L;Li M;Shen M;Zhou Z;Zhu S;Li K;Fang Z;Yan B;Zhao S;Su J;Chen X;Peng C
研究背景Exosomes具有无创性、低成本、敏感性高、在体液中稳定等优点，是一种很有前途的疾病检测工具。microRNA（miRNAs）是外泌体的主要成分，参与肿瘤的发生发展。然而，亚洲黑色素瘤patients.MethodsExosomal miRNA的轮廓之间仍然不清楚从黑色素瘤患者（n = 20）和健康个体（n = 20）的血浆Exosomal miRNA的分离和进行小RNA测序。采用实时荧光定量PCR技术对差异表达的miRNAs进行鉴定，以确定其诊断效率。结果Exosomal miRNA表达谱显示miR-1180- 3 p表达降低，可作为黑色素瘤的潜在诊断标志物。黑色素瘤患者（n = 28）和对照（n = 28）的验证组证实了miR-1180- 3 p的诊断效率。miR-1180- 3 p在黑色素瘤细胞中的表达水平低于黑素细胞。因此，miR-1180- 3 p的水平与黑色素瘤细胞的增殖、迁移和侵袭呈负相关。功能分析和靶基因预测发现，ST 3GAL 4是一个潜在的靶基因，在黑色素瘤组织中高表达，并受miR-1180- 3 p负调控。敲低ST 3GAL 4阻碍了恶性表型的黑色素瘤cells.ConclusionsThis study indicates that reduced exosomal miR-1180- 3 p in melanoma patient plasma is a potential diagnosis marker and provides novel insight into melanoma development.
BackgroundExosomes are a promising tool in disease detection because they are noninvasive, cost-effective, sensitive and stable in body fluids. MicroRNAs (miRNAs) are the main exosomal component and participate in tumor development. However, the exosomal miRNA profile among Asian melanoma patients remains unclear.MethodsExosomal miRNAs from the plasma of melanoma patients (n = 20) and healthy individuals (n = 20) were isolated and subjected to small RNA sequencing. Real-time PCR was performed to identify the differential miRNAs and to determine the diagnostic efficiency. Proliferation, scratch and Transwell assays were performed to detect the biological behavior of melanoma cells.ResultsExosomal miRNA profiling revealed decreased miR-1180-3p expression as a potential diagnostic marker of melanoma. The validation group of melanoma patients (n = 28) and controls (n = 28) confirmed the diagnostic efficiency of miR-1180-3p. The level of miR-1180-3p in melanoma cells was lower than that in melanocytes. Accordingly, the level of miR-1180-3p was negatively associated with the proliferation, migration and invasion of melanoma cells. Functional analysis and target gene prediction found that ST3GAL4 was a potential target and highly expressed in melanoma tissues and was negatively regulated by miR-1180-3p. Knockdown of ST3GAL4 hindered the malignant phenotype of melanoma cells.ConclusionsThis study indicates that reduced exosomal miR-1180-3p in melanoma patient plasma is a promising diagnostic marker and provides novel insight into melanoma development.
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DOI：
10.1126/science.aau6977
发表时间：
2020-02-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kalluri R;LeBleu VS
通讯作者：
LeBleu VS
影响因子：
7
作者：
Lee, Inhan;Ajay, Subramanian S.;Athey, Brian D.
通讯作者：
Athey, Brian D.
影响因子：
3.8
作者：
Chi Z;Li S;Sheng X;Si L;Cui C;Han M;Guo J
通讯作者：
Guo J
影响因子：
2.6
作者：
Macfarlane LA;Murphy PR
通讯作者：
Murphy PR
影响因子：
14.9
作者：
Pathan M;Fonseka P;Chitti SV;Kang T;Sanwlani R;Van Deun J;Hendrix A;Mathivanan S
通讯作者：
Mathivanan S