Surface Immunoglobulin Light Chain Expression in Pre‐B Cell Leukemias a
Surface Immunoglobulin Light Chain Expression in Pre‐B Cell Leukemias a
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前 B 细胞白血病中的表面免疫球蛋白轻链表达
DOI：
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发表时间：
1986
影响因子：
5.2
通讯作者：
B. Clarkson
中科院分区：
文献类型：
--
作者：
B. Koziner;J. Stavnezer;A. Al;D. Gebhard;A. Mittelman;M. Andreeff;B. Clarkson
对32例白血病患者单个核细胞表面免疫球蛋白(Sig)轻链克隆性(CE)进行了细胞荧光分析，定义为(%kappa+-%lambda+)/(%kappa++%lambda+)细胞。8例表现为SIG轻链CE，其中4例为慢性髓系白血病(BLCML)，3例为“零”急性淋巴细胞白血病(ALL)，1例为白血病淋巴母细胞性淋巴瘤。其中6例白血病表现为kappa CE，2例为lambda CE。从一例BL-CML患者分离的kappa+PB细胞被证明具有二倍体DNA干系，并携带“共同的”ALL抗原。为了进一步支持我们发现SIG轻链在ALL中的表达，我们研究了来自一名“普通”ALL患者的REH细胞系，发现了细胞质MU重链和表面Ig lambda CE。对REH的核酸印迹实验表明，在表达lambda轻链的B细胞中，两个kappa基因都被删除了，lambda基因发生了重排。此外，REH细胞含有u和lambda RNA。当用TPA处理REH细胞时，Mu链RNA的量增加了约5倍，而lambda链RNA的量增加了约2倍。在前B细胞白血病和REH细胞系中SIG轻链的发现表明，这些白血病细胞沿着B细胞谱系比先前认为的更进一步分化。
Cytofluorographic analysis of surface immunoglobulin (sIg) light chain clonal excess (CE), defined as (%kappa+ - %lambda+)/(%kappa+ + %lambda+) cells per discrete level of fluorescence intensity, was carried out on mononuclear cells of 32 leukemic patients. Eight demonstrated sIg light chain CE, including four blastic chronic myeloid leukemias (BL-CML), three "null" acute lymphoblastic leukemias (ALL), and one leukemic lymphoblastic lymphoma. Six of the leukemias demonstrated a kappa CE and two had a lambda CE. Sorted kappa+ PB cells from a BL-CML patient were shown to have a diploid DNA stem line and to bear the "common" ALL antigen. To provide further support for our finding of the expression of sIg light chains in ALL, we studied the REH cell line, derived from a "common" ALL patient and found cytoplasmic mu heavy chain and surface Ig lambda CE. Nucleic acid blotting experiments on REH revealed that both kappa genes had been deleted and that lambda genes had been rearranged, as expected in B cells expressing lambda light chains. Moreover, REH cells contained mu and lambda RNA. When REH cells were treated with TPA the amount of mu chain RNA increased by approximately fivefold and the amount of lambda chain RNA increased by approximately twofold. The finding of sIg light chain in pre-B cell leukemias and in the REH cell line, suggests that these leukemic cells are further differentiated along the B-cell lineage than was previously believed.
影响因子：
15.9
作者：
NADLER, LM;RITZ, J;STASHENKO, P
通讯作者：
STASHENKO, P
非 T 细胞急性淋巴细胞白血病中人类 B 细胞抗原的诱导。
DOI：
10.1172/jci110633
发表时间：
1982
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Nadler,LM;Ritz,J;Bates,MP;Park,EK;Anderson,KC;Sallan,SE;Schlossman,SF
通讯作者：
Schlossman,SF
佛波酯诱导的非 T、非 B 白血病细胞系分化：人淋巴祖细胞发育模型。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
LeBien,TW;Bollum,FJ;Yasmineh,WG;Kersey,JH
通讯作者：
Kersey,JH