Contractile acto-myosin network on nuclear envelope remnants positions human chromosomes for mitosis
Contractile acto-myosin network on nuclear envelope remnants positions human chromosomes for mitosis
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核膜残余物上的收缩肌动球蛋白网络定位人类染色体有丝分裂
DOI：
10.1101/459750
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Booth A
中科院分区：
文献类型：
--
作者：
Booth A
为了确保有丝分裂过程中的适当分离，染色体必须被纺锤体微管有效地捕获并随后在有丝分裂纺锤体上排列。染色体与纺锤体相互作用的效果可能受到染色体在空间中分散的程度的影响。在这里，我们量化了染色体散射体积（CSV），发现它在人类细胞核膜分解（NEBD）后很快就减少了。CSV减少主要独立于微管发生，因此不是染色体和纺锤体之间相互作用的结果。我们发现，在NEBD之前，一个肌动蛋白网络以LINC复合物依赖的方式组装在核膜的细胞质表面。NEBD发生后不久，肌凝蛋白-肌球蛋白网络仍保留在核膜残余物上，其肌凝蛋白ii介导的收缩减少了CSV，促进了及时的染色体聚集和正确的分离。因此，我们发现了一种新的机制，在早期有丝分裂中定位染色体，以确保有效和正确的染色体-纺锤体相互作用。
To ensure proper segregation during mitosis, chromosomes must be efficiently captured by spindle microtubules and subsequently aligned on the mitotic spindle. The efficacy of chromosome interaction with the spindle can be influenced by how widely chromosomes are scattered in space. Here, we quantify chromosome-scattering volume (CSV) and find that it is reduced soon after nuclear envelope breakdown (NEBD) in human cells. The CSV reduction occurs primarily independently of microtubules and is therefore not an outcome of interactions between chromosomes and the spindle. We find that, prior to NEBD, an acto-myosin network is assembled in a LINC complex-dependent manner on the cytoplasmic surface of the nuclear envelope. This acto-myosin network remains on nuclear envelope remnants soon after NEBD, and its myosin-II-mediated contraction reduces CSV and facilitates timely chromosome congression and correct segregation. Thus, we find a novel mechanism that positions chromosomes in early mitosis to ensure efficient and correct chromosome–spindle interactions.
DOI：
10.1073/pnas.1202786109
发表时间：
2012-06-12
影响因子：
11.1
作者：
Kepiro, Miklos;Varkuti, Boglarka H.;Malnasi-Csizmadia, Andras
通讯作者：
Malnasi-Csizmadia, Andras
肌动蛋白和肌球蛋白在激光微束穿过纺锤体照射引起的 PtK2 纺锤体长度变化中的作用
影响因子：
2.9
作者：
R. Sheykhani;N. Baker;Veronica Gomez‐Godinez;L. Liaw;J. Shah;M. Berns;A. Forer
通讯作者：
A. Forer