Endothelial cell micropatterning: methods, effects, and applications.

Endothelial cell micropatterning: methods, effects, and applications.
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DOI:
10.1007/s10439-011-0352-z
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发表时间:
2011-09
影响因子:
3.8
通讯作者:
Hinds, Monica T.
Hinds, Monica T.
中科院分区:
工程技术2区
文献类型:
--
作者:
Anderson, Deirdre E. J.;Hinds, Monica T.

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The effects of flow on endothelial cells have been widely examined for the ability of fluid shear stress to alter cell morphology and function; however, the effects of endothelial cell morphology without flow have only recently been observed. An increase in lithographic techniques in cell culture spurred a corresponding increase in research aiming to confine cell morphology. These studies lead to a better understanding of how morphology and cytoskeletal configuration affect the structure and function of the cells. This review examines endothelial cell micropatterning research by exploring both the many alternative methods used to alter endothelial cell morphology and the resulting changes in cellular shape and phenotype. Micropatterning induced changes in endothelial cell proliferation, apoptosis, cytoskeletal organization, mechanical properties, and cell functionality. Finally, the ways these cellular manipulation techniques have been applied to biomedical engineering research, including angiogenesis, cell migration, and tissue engineering, is discussed.
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