Circulating serum trefoil factor 3 (TFF3) is dramatically increased in chronic kidney disease.
Circulating serum trefoil factor 3 (TFF3) is dramatically increased in chronic kidney disease.
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DOI：
10.1371/journal.pone.0080271
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Du TY;Luo HM;Qin HC;Wang F;Wang Q;Xiang Y;Zhang Y
三叶因子3(TFF3)是一种在粘膜保护、细胞增殖和细胞迁移中发挥重要作用的小肽。TFF3的异常表达与胃肠道炎症、实体瘤等临床疾病密切相关。本研究的目的是了解血清TFF3在常见临床疾病中的分布特点。采用酶联免疫吸附试验(ELISA)对1072名中国患者进行了一项大型前瞻性随机研究，以检测不同疾病患者的血清TFF3浓度。对慢性肾脏病(CKD)1~5期患者进行配对病例对照研究。采用免疫组织化学(IHC)方法，观察血清和尿液中TFF3多肽浓度与CKD病情严重程度的关系。慢性肾脏病、转移及继发性肿瘤和急性胃肠炎患者的血清TFF3平均浓度分别为200.9 ng/ml、95.7 ng/ml和71.7 ng/ml，均显著高于其他临床常见病患者。血清TFF3浓度与CKD病情严重程度呈正相关趋势。CKD 1~5期患者血清TFF3值分别为23.6 ng/ml、29.9 ng/ml、54.9 ng/ml、85.0 ng/ml和176.6 ng/ml。尿TFF3浓度和CKD分期也有相同的变化趋势。CKD1~5期尿肌酐校正浓度分别为367.1 ng/mg·Cr、910.6 ng/mg·Cr、1,149.0 ng/mg·Cr、1,610.0 ng/mg·Cr和3,475.0 ng/mg·Cr。免疫组化显示TFF3主要表达于肾小管上皮细胞。在进行临床TFF3研究时，必须充分考虑肾脏损伤的影响。对TFF3在慢性肾脏病中的进一步研究将有助于我们了解其在其他疾病中的病理作用和机制。
Trefoil factor 3 (TFF3) is a small peptide that plays an important role in mucosal protection, cell proliferation, and cell migration. The aberrant expression of TFF3 is correlated with gastrointestinal inflammation, solid tumors, and other clinical diseases. The objective of this study was to identify the distribution characteristics of serum TFF3 in common clinical diseases. A large prospective randomized study of 1,072 Chinese patients was performed using an enzyme-linked immunosorbent assay (ELISA) to examine the serum TFF3 concentrations in patients with different diseases. A matched case-control study was conducted on patients with chronic kidney disease (CKD) stages 1–5. Immunohistochemistry (IHC) was performed using renal tissues to determine the relationship between the severity of CKD and the serum and urine concentrations of TFF3 peptides. The mean serum concentrations of TFF3 in patients with CKD, metastatic and secondary carcinoma (MC) and acute gastroenteritis (AG) (200.9 ng/ml, 95.7 ng/ml and 71.7 ng/ml, respectively) were significantly higher than those in patients with other common clinical diseases. A positive correlation tendency was observed between the serum TFF3 concentrations and the severity of CKD. The mean serum TFF3 values for CKD stages 1–5 were 23.6 ng/ml, 29.9 ng/ml, 54.9 ng/ml, 85.0 ng/ml and 176.6 ng/ml, respectively. The same trend was observed in the urine TFF3 concentrations and the CKD stages. The creatinine(Cr)-corrected concentrations of TFF3 in urine were 367.1 ng/mg·Cr, 910.6 ng/mg·Cr, 1,149.0 ng/mg·Cr, 1,610.0 ng/mg·Cr and 3,475.0 ng/mg·Cr for CKD stages 1–5, respectively. IHC revealed that TFF3 expression was concentrated in tubular epithelial cells. The influence of kidney injuries must be fully considered when performing clinical TFF3 research. Further studies on TFF3 in CKD will contribute to our understanding of its pathological roles and mechanisms in other diseases.
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